Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature

Tandrup, Tobias; Leggio, Leila Lo; Meilleur, Flora

doi:10.1107/s2053230x22011335

Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature

Journal Article · Sun Jan 01 00:00:00 EST 2023 · Acta Crystallographica. Section F, Structural Biology Communications

DOI:https://doi.org/10.1107/s2053230x22011335· OSTI ID:2471398

Tandrup, Tobias ^[1]; ^[1]; ^[2]

University of Copenhagen (Denmark)
North Carolina State University, Raleigh, NC (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Lytic polysaccharide monooxygenases (LPMOs) are copper metalloenzymes which cleave polysaccharides oxidatively and are important in pathogen biology, carbon cycling and biotechnology. The Lentinus similis family AA9 isoform A (LsAA9_A) has been extensively studied as a model system because its activity towards smaller soluble saccharide substrates has allowed detailed structural characterization of its interaction with a variety of substrates by X-ray crystallography at high resolution. Here, the joint X-ray/neutron room-temperature crystallographic structure of carbohydrate-free LsAA9_A in the copper(II) resting state refined against X-ray and neutron data at 2.1 and 2.8 Å resolution, respectively, is presented. The results provide an experimental determination of the protonation states of the copper(II)-coordinating residues and second-shell residues in LsAA9_A, paving the way for future neutron crystallographic studies of LPMO–carbohydrate complexes.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: Danish Council for Independent Research; Novo Nordisk Foundation; USDA; USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 2471398

Journal Information:: Acta Crystallographica. Section F, Structural Biology Communications, Journal Name: Acta Crystallographica. Section F, Structural Biology Communications Journal Issue: 1 Vol. 79; ISSN 2053-230X

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Lentinus similis AA9_A
biochemistry & molecular biology
biophysics
copper metalloenzymes
crystallography
lytic polysaccharide monooxygenases
neutron crystallography
protonation states

Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature

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