Insights into an unusual Auxiliary Activity 9 family member lacking the histidine brace motif of lytic polysaccharide monooxygenases

Frandsen, Kristian E.  H.; Tovborg, Morten; Jørgensen, Christian I.; Spodsberg, Nikolaj; Rosso, Marie-Noëlle; Hemsworth, Glyn R.; Garman, Elspeth F.; Grime, Geoffrey W.; Poulsen, Jens-Christian N.; Batth, Tanveer S.; Miyauchi, Shingo; Lipzen, Anna; Daum, Chris; Grigoriev, Igor V.; Johansen, Katja S.; Henrissat, Bernard; Berrin, Jean-Guy; Lo Leggio, Leila

doi:10.1074/jbc.ra119.009223

Insights into an unusual Auxiliary Activity 9 family member lacking the histidine brace motif of lytic polysaccharide monooxygenases

Journal Article · Fri Aug 30 00:00:00 EDT 2019 · Journal of Biological Chemistry

DOI:https://doi.org/10.1074/jbc.ra119.009223· OSTI ID:1619137

^[1]; Tovborg, Morten ^[2]; Jørgensen, Christian I. ^[2]; Spodsberg, Nikolaj ^[2]; Rosso, Marie-Noëlle ^[3]; Hemsworth, Glyn R. ^[4]; ^[5]; ^[6]; ^[7]; Batth, Tanveer S. ^[7]; ^[3]; Lipzen, Anna ^[8]; Daum, Chris ^[8]; Grigoriev, Igor V. ^[9]; Johansen, Katja S. ^[10]; Henrissat, Bernard ^[11]; Berrin, Jean-Guy ^[3]; ^[7]

Univ. of Copenhagen (Denmark); Aix-Marseille Univ., Marseille (France)
Novozymes, Bagsvaerd (Denmark)
Aix-Marseille Univ., Marseille (France)
Univ. of Leeds (United Kingdom); Univ. of York (United Kingdom)
Univ. of Oxford (United Kingdom)
Univ. of Surrey, Guildford (United Kingdom)
Univ. of Copenhagen (Denmark)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Univ. of California, Berkeley, CA (United States)
Univ. of Copenhagen (United Kingdom)
Aix-Marseille Univ., Marseille (France); INRA, Marseille (France); King Abdulaziz Univ., Jeddah (Saudi Arabia)

Lytic polysaccharide monooxygenases (LPMOs) are redox-enzymes involved in biomass degradation. All characterized LPMOs possess an active site of two highly conserved histidine residues coordinating a copper ion (the histidine brace), which are essential for LPMO activity. However, some protein sequences that belong to the AA9 LPMO family display a natural N-terminal His to Arg substitution (Arg-AA9). These are found almost entirely in the phylogenetic fungal class Agaricomycetes, associated with wood decay, but no function has been demonstrated for any Arg-AA9. Through bioinformatics, transcriptomic, and proteomic analyses we present data, which suggest that Arg-AA9 proteins could have a hitherto unidentified role in fungal degradation of lignocellulosic biomass in conjunction with other secreted fungal enzymes. We present the first structure of an Arg-AA9, LsAA9B, a naturally occurring protein from Lentinus similis The LsAA9B structure reveals gross changes in the region equivalent to the canonical LPMO copper-binding site, whereas features implicated in carbohydrate binding in AA9 LPMOs have been maintained. We obtained a structure of LsAA9B with xylotetraose bound on the surface of the protein although with a considerably different binding mode compared with other AA9 complex structures. In addition, we have found indications of protein phosphorylation near the N-terminal Arg and the carbohydrate-binding site, for which the potential function is currently unknown. Finally, our results are strong evidence that Arg-AA9s function markedly different from canonical AA9 LPMO, but nonetheless, may play a role in fungal conversion of lignocellulosic biomass.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: Biotechnology and Biotechnology Sciences (BBSRC); Carlsberg Foundation; Danish Council for Strategic Research; Danish Ministry of Higher Education and Science; Novo Nordisk Foundation; USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1619137

Journal Information:: Journal of Biological Chemistry, Journal Name: Journal of Biological Chemistry Journal Issue: 45 Vol. 294; ISSN 0021-9258

Publisher:: American Society for Biochemistry and Molecular BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Insights into an unusual Auxiliary Activity 9 family member lacking the histidine brace motif of lytic polysaccharide monooxygenases

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