Analysis of phospholipids and triacylglycerols in intravenous lipid emulsions

Banstola, Bijay; Gamage, Prabhath L.; Jiang, Wenlei; Mudalige, Thilak

doi:10.1016/j.jpba.2022.115112

Analysis of phospholipids and triacylglycerols in intravenous lipid emulsions

Journal Article · Tue Oct 18 00:00:00 EDT 2022 · Journal of Pharmaceutical & Biomedical Analysis

DOI:https://doi.org/10.1016/j.jpba.2022.115112· OSTI ID:2425847

Banstola, Bijay ^[1]; Gamage, Prabhath L. ^[1]; Jiang, Wenlei ^[2]; Mudalige, Thilak ^[1]

US Food and Drug Administration, Jefferson, AR (United States)
US Food and Drug Administration, Silver Spring, MD (United States)

Intravenous lipid emulsions (ILEs) are used for parenteral nutrition, providing a vital source of essential fatty acids and concentrated energy for patients who are unable to absorb nutrients via the digestive track. They are commonly used to treat local and non-local anesthetic toxicity, and lipophilic drug overdose. ILE are composed of natural lipids, and the composition of these natural lipids can be varied based on their source. The lipids are susceptible to hydrolytic degradation with time, resulting various lipid degradation products such as Lysophosphatidylcholines (LPs), affecting the actual composition of nutrients in the formulation. As a result, the identification and quantification of lipid components, including degradation products, in ILEs are crucial in quality control. In this study, lipids from different batches of ILE Intralipid® 20%, were separated and identified using a UHPLC-ESI-QTOF system and SimLipid® high throughput lipid identification software. Out of 47 lipids identified, 34 were phospholipids (PLs) and the others were triacylglycerols (TAGs). Most of the phospholipids detected were phosphatidylcholines (PC) and Lysophosphatidylcholines (LPC). A total of 9 LPCs, 18 PCs, 6 phosphoethanolamines (PEs), and 1 sphingomyelin (SM) were identified. The LPCs concentration changed with the manufacturing date and storage time. Furthermore, this UHPLC method enabled the identification and quantification of lipids and their decomposition products in complex ILE emulsion mixtures on a single 20-minute chromatographic run.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0014664

OSTI ID:: 2425847

Journal Information:: Journal of Pharmaceutical & Biomedical Analysis, Journal Name: Journal of Pharmaceutical & Biomedical Analysis Journal Issue: C Vol. 222; ISSN 0731-7085

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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60 APPLIED LIFE SCIENCES
Chemistry
Intravenous lipid emulsions
LCMS
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Triacylglycerides
UHPLC

Analysis of phospholipids and triacylglycerols in intravenous lipid emulsions

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References (37)

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