Engineering Caldicellulosiruptor bescii with Surface Layer Homology Domain-Linked Glycoside Hydrolases Improves Plant Biomass Solubilization

Laemthong, Tunyaboon; Bing, Ryan G.; Crosby, James R.; Adams, Michael W.  W.; Kelly, Robert M.; Alexandre, Gladys

doi:10.1128/aem.01274-22

Engineering Caldicellulosiruptor bescii with Surface Layer Homology Domain-Linked Glycoside Hydrolases Improves Plant Biomass Solubilization

Journal Article · Wed Oct 26 00:00:00 EDT 2022 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/aem.01274-22· OSTI ID:2422499

Laemthong, Tunyaboon ^[1]; Bing, Ryan G. ^[2]; Crosby, James R. ^[2]; ^[3]; ^[2]; Alexandre, Gladys

Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina, USA; OSTI
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina, USA
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA

Caldicellulosiruptorspecies hold promise as microorganisms that can solubilize the carbohydrate portion of lignocellulose and subsequently convert fermentable sugars into bio-based chemicals and fuels. Members of the genus have surface layer (S-layer) homology domain-associated glycoside hydrolases (SLH-GHs) that mediate attachment to biomass as well as hydrolysis of carbohydrates.Caldicellulosiruptor bescii, the most studied member of the genus, has only one SLH-GH.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0019391; SC0022192

OSTI ID:: 2422499

Journal Information:: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 20 Vol. 88; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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