Novel multidomain, multifunctional glycoside hydrolases from highly lignocellulolytic Caldicellulosiruptor species

Conway, Jonathan M.; Crosby, James R.; Hren, Andrew P.; Southerland, Robert T.; Lee, Laura L.; Lunin, Vladimir V.; Alahuhta, Petri; Himmel, Michael E.; Bomble, Yannick J.; Adams, Michael W.  W.; Kelly, Robert M.

doi:10.1002/aic.16354

Novel multidomain, multifunctional glycoside hydrolases from highly lignocellulolytic Caldicellulosiruptor species

Journal Article · Wed Jul 11 00:00:00 EDT 2018 · AIChE Journal

DOI:https://doi.org/10.1002/aic.16354· OSTI ID:1475518

Conway, Jonathan M. ^[1]; Crosby, James R. ^[1]; Hren, Andrew P. ^[1]; Southerland, Robert T. ^[1]; Lee, Laura L. ^[1]; Lunin, Vladimir V. ^[2]; Alahuhta, Petri ^[2]; Himmel, Michael E. ^[2]; Bomble, Yannick J. ^[2]; Adams, Michael W. W. ^[3]; ^[1]

North Carolina State Univ., Raleigh, NC (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Univ. of Georgia, Athens, GA (United States)

Biological hydrolysis of microcrystalline cellulose is an uncommon feature in the microbial world, especially among bacteria and archaea growing optimally above 70 degrees C (the so-called extreme thermophiles). In fact, among this group only certain species in the genus Caldicellulosiruptor are capable of rapid and extensive cellulose degradation. Four novel multidomain glycoside hydrolases (GHs) from Caldicellulosiruptor morganii and Caldicellulosiruptor danielii were produced recombinantly in Caldicellulosiruptor bescii and characterized. These GHs are structurally organized with two or three catalytic domains flanking carbohydrate binding modules from Family 3. Collectively, these enzymes represent GH families 5, 9, 10, 12, 44, 48, and 74, and hydrolyze crystalline cellulose, glucan, xylan, and mannan, the primary carbohydrates in plant biomass. Degradation of microcrystalline cellulose by cocktails of GHs from three Caldicellulosiruptor species demonstrated that synergistic interactions enable mixtures of multiple enzymes to outperform single enzymes, suggesting a community mode of action for lignocellulose utilization in thermal environments.

View Accepted Manuscript (DOE)

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1475518

Alternate ID(s):: OSTI ID: 1464583

Report Number(s):: NREL/JA--2700-71961

Journal Information:: AIChE Journal, Journal Name: AIChE Journal Journal Issue: 12 Vol. 64; ISSN 0001-1541

Publisher:: American Institute of Chemical EngineersCopyright Statement

Country of Publication:: United States

Language:: English

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Novel multidomain, multifunctional glycoside hydrolases from highly lignocellulolytic Caldicellulosiruptor species

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