Genomic organization of the mouse cyclin D1 gene (Cyl-1)
- Imperial Cancer Research Fund Laboratories, London (United Kingdom)
To determine the genomic organization of the mouse cyclin D1 locus (Cyl-1), a series of cosmids and cDNAs were recovered by hybridization with a genomic probe representing the 5{prime} end of the homologous human gene, CCND1. Primer extension indicated that transcripts originate from one of three adjacent nucleotides at a single start site. Two overlapping cDNA clones that essentially accounted for the complete sequence of the larger 4.0-kb Cyl-1 transcript were characterized. A combination of RNase protection and sequencing across intron-exon boundaries established that the gene is organized into five coding exons with a long 3{prime} untranslated region. Repeated attempts to isolate clones corresponding to the minor 3.5-kb RNA were compromised by the presence of an internal poly(A) domain. However, hybridization with specific probes revealed that the minor transcript lacks approximately 800 nucleotides from the 3{prime} end of the major transcript and may be generated by a novel mechanism or by RNA processing. 27 refs., 5 figs., 1 tab.
- OSTI ID:
- 241069
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 25; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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