Chromosomal location and some structural features of human clathrin light-chain genes (CLTA and CLTB)

Journal Article · Thu Dec 01 04:00:00 EST 1994 · Genomics

DOI:https://doi.org/10.1006/geno.1994.1650· OSTI ID:241030

Ponnambalam, S ^[1]; Pravtcheva, D; Ruddle, F H ^[2]

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1. Imperical Cancer Research Fund, London (United Kingdom)
2. Yale Univ., New Haven, CT (United States); and others

Two human clathrin light-chain genes have been defined. The gene (CLTA) encoding the LCa light chain maps to the long arm of chromosome 12 at 12q23-q24 and that encoding the LCb light chain (CLTB) maps to the long arm of chromosome 4 at 4q2-q3. Isolation and characterization of partial genomic clones encoding human LCa and LCb reveal the neuron-specific insertions of the LCa and LCb proteins to be encoded by discrete exons, thus proving that clathrin light chains undergo alternate mRNA splicing to generate tissue-specific protein isoforms. The insertion sequence of LCb is encoded by a single exon and that of LCa by two exons. The first of the two neuron-specific LCa exons is homologous to the corresponding LCb exon. An intronic sequence of the LCb gene with similarity to the second neuron-specific exon of the LCa gene has been identified. 31 refs., 2 figs., 2 tabs.

• OSTI ID: 241030
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 24; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA HYBRIDIZATION
DNA-CLONING
EXONS
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 12
HUMAN CHROMOSOMES
HYBRIDIZATION
MESSENGER-RNA
PROBES
PROTEINS
SOMATIC CELLS
SPLICING
STRUCTURE-ACTIVITY RELATIONSHIPS