Localization of a clathrin heavy chain gene to chromosome 22

Journal Article · Thu Sep 01 04:00:00 EDT 1994 · American Journal of Human Genetics

OSTI ID:134810

Long, K; Buckler, A; Trofatter, J ^[1]

---

1. Massachusetts General Hospital, Boston (United States)

Random isolation of exons from human chromosome 22 using the exon amplification procedure has identified a number of sequences having similarity to previously cloned genes. One exon was found to be highly similar to clathrin heavy chain genes. Previously, a putative clathrin heavy chain gene has been mapped to chromosome 17, but comparison of the predicted amino acid sequence of this gene to the other clathrin genes in public databases indicates that there is little or no similarity between the chromosome 17 gene and the others. The exon isolated from chromosome 22 shows significant similarity to the other clathrin genes but not the chromosome 17 sequence. This high level of peptide similarity extends even to the Drosophila melanogaster clathrin gene. Somatic cell hybrids containing different portions of chromosome 22 localize the gene above D22S193 placing it within the 22p12-q11 region, which is a particularly disease-rich region of the chromosome. A YAC and cosmid contig has been constructed surrounding the gene and isolation of a full-length cDNA is in progress. The localization of this gene is especially important given the role of clathrin with regards to receptor-mediated endocytosis and signal transduction.

• OSTI ID: 134810
• Report Number(s): CONF-941009--
• Journal Information: American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
BIOLOGICAL MARKERS
COMPARATIVE EVALUATIONS
CONTIGS
DESIGN
DISEASES
DNA SEQUENCING
DNA-CLONING
DROSOPHILA
EXONS
GENE AMPLIFICATION
GENES
GENETIC MAPPING
GENETICS
HUMAN CHROMOSOME 17
HUMAN CHROMOSOME 22
HYBRIDIZATION
PEPTIDES
SOMATIC CELLS
STRUCTURE-ACTIVITY RELATIONSHIPS
YEASTS