High resolution transcription map from D22S56 to D22S268 in the 22q12 region surrounding the NF2 gene

Journal Article · Thu Sep 01 04:00:00 EDT 1994 · American Journal of Human Genetics

OSTI ID:134487

Trofatter, J; Long, K; Murrell, J ^[1]

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1. Massachusetts General Hospital, Charlestown, MA (United States); and others

Exon amplification is an efficient method to extract exons from stretches of genomic DNA and is especially proficient when single or small pools of cosmids are used as the starting material. YACs spanning the region from D22S56 to D22S268 were identified from the CEPH1 YAC library using a combination of Alu-PCR hybridization and STS PCR. The minimal YAC contig was then used to identify cosmids from the LL22NC03 chromosome 22-specific cosmid library. Small pools of cosmids (5-10) were subjected to the exon amplification procedure. The exons were hybridized back to the YACs and cosmids simultaneously constructing an order cloned contig and a transcription map. The genes previously known to reside in this region (EWSR1, NEHF, NF2) were identified as well as a number of new genes which will be described. This study demonstrates that the described approach is generally applicable for saturation of any genomic region with gene sequences.

• OSTI ID: 134487
• Report Number(s): CONF-941009--
• Journal Information: American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CONTIGS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
EXONS
GENE AMPLIFICATION
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 22
POLYMERASE CHAIN REACTION
TRANSCRIPTION
YEASTS