Characterization of the expressed CIITA allele in the class II MHC transcriptional mutant RJ2.2.5

Journal Article · Sat Jun 01 04:00:00 EDT 1996 · Immunogenetics

OSTI ID:240997

Brown, J A; He, X F; Westerheide, S D ^[1]

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1. Emory Univ. School of Medicine, Atlanta, GA (United States); and others

Class II major histocompatibility complex (MHC) genes are regulated in a coordinate manner. A set of conserved upstream elements termed the W/Z/S, X1, X2, and Y boxes are found 5{prime} to class II genes, the R gene, and the HLA-DM genes. These conserved elements are required for tissue-specific and IFN{gamma}-mediated regulation of these genes. The DNA-binding proteins RFX, X2BP, and NFY have been found to specifically interact with the X1, X2, and Y box elements, respectively, as well as with each other. A role for an additional factor was recently demonstrated by the cloning of a gene that could complement the MHC class H gene-specific transcriptional deficiency in the mutant cell line RJ2.2.5 as well as cell lines isolated from patients exhibiting the bare lymphocyte syndrome. This gene was termed the class II transactivator or CIITA. While both genetic and biochemical studies have indicated interactions between the DNA-binding proteins described above, direct interactions with CIITA have not been described. 23 refs., 3 figs.

• OSTI ID: 240997
• Journal Information: Immunogenetics, Journal Name: Immunogenetics Journal Issue: 1-2 Vol. 43; ISSN IMNGBK; ISSN 0093-7711
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ANIMAL CELLS
CODONS
DNA-CLONING
GENE MUTATIONS
GENE REGULATION
GENES
HISTOCOMPATIBILITY COMPLEX
MUTANTS
POLYMERASE CHAIN REACTION
PROTEINS
TRANSCRIPTION