Developmental Ethanol Exposure Impacts Purkinje Cells but Not Microglia in the Young Adult Cerebellum

Cealie, MaKenna Y.; Douglas, James C.; Swan, Hannah K.; Vonkaenel, Erik D.; McCall, Matthew N.; Drew, Paul D.; Majewska, Ania K.

doi:10.3390/cells13050386

Developmental Ethanol Exposure Impacts Purkinje Cells but Not Microglia in the Young Adult Cerebellum

Journal Article · Fri Feb 23 00:00:00 EST 2024 · Cells

DOI:https://doi.org/10.3390/cells13050386· OSTI ID:2407283

^[1]; Douglas, James C. ^[2]; Swan, Hannah K. ^[1]; Vonkaenel, Erik D. ^[3]; McCall, Matthew N. ^[1]; ^[2]; ^[1]

University of Rochester, NY (United States)
University of Arkansas for Medical Sciences, Little Rock, AR (United States)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Fetal alcohol spectrum disorders (FASD) caused by developmental ethanol exposure lead to cerebellar impairments, including motor problems, decreased cerebellar weight, and cell death. Alterations in the sole output of the cerebellar cortex, Purkinje cells, and central nervous system immune cells, microglia, have been reported in animal models of FASD. To determine how developmental ethanol exposure affects adult cerebellar microglia and Purkinje cells, we used a human third-trimester binge exposure model in which mice received ethanol or saline from postnatal (P) days 4–9. In adolescence, cerebellar cranial windows were implanted and mice were aged to young adulthood for examination of microglia and Purkinje cells in vivo with two-photon imaging or in fixed tissue. Ethanol had no effect on microglia density, morphology, dynamics, or injury response. However, Purkinje cell linear frequency was reduced by ethanol. Microglia–Purkinje cell interactions in the Purkinje Cell Layer were altered in females compared to males. Overall, developmental ethanol exposure had few effects on cerebellar microglia in young adulthood and Purkinje cells appeared to be more susceptible to its effects.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: Eunice Kennedy Shriver National Institute of Child Health and Human Development; National Institute on Alcohol Abuse and Alcoholism (NIAAA); National Institutes of Health (NIH); USDOE; University of Rochester Intellectual and Developmental Disabilities Research Center

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 2407283

Report Number(s):: PNNL-SA--194532

Journal Information:: Cells, Journal Name: Cells Journal Issue: 5 Vol. 13; ISSN 2073-4409

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Purkinje cell
cerebellum
ethanol
fetal alcohol spectrum disorders
immune system
microglia
neurodevelopment
prenatal alcohol exposure
two-photon imaging

Developmental Ethanol Exposure Impacts Purkinje Cells but Not Microglia in the Young Adult Cerebellum

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