Oligodendrocyte ablation affects the coordinated interaction between granule and Purkinje neurons during cerebellum development

Journal Article · Wed Aug 01 04:00:00 EDT 2007 · Experimental Cell Research

DOI:https://doi.org/10.1016/j.yexcr.2007.05.003· OSTI ID:20972096

Collin, Ludovic ^[1]; Doretto, Sandrine ^[1]; Malerba, Monica ^[1]; Ruat, Martial ^[2]; Borrelli, Emiliana ^[3]

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1. Institut de Genetique et de Biologie Moleculaire et Cellulaire, 1 rue L. Fries 67404 Illkirch (France)
2. Institut de Neurobiologie Alfred Fessard, IFR 2118 CNRS, Signal Transduction and Developmental Neuropharmacology, CNRS UPR9040, 1 Avenue de la Terrasse, 91198 Gif sur Yvette (France)
3. Institut de Genetique et de Biologie Moleculaire et Cellulaire, 1 rue L. Fries 67404 Illkirch (France) and Department of Psychiatry and Human Behavior, University of California Irvine, 3226 Gillespie Neuroscience Research Facility, Irvine CA 92697 (United States)

Oligodendrocytes (OLs) are the glial cells of the central nervous system (CNS) classically known to be devoted to the formation of myelin sheaths around most axons of the vertebrate brain. We have addressed the role of these cells during cerebellar development, by ablating OLs in vivo. Previous analyses had indicated that OL ablation during the first six postnatal days results into a striking cerebellar phenotype, whose major features are a strong reduction of granule neurons and aberrant Purkinje cells development. These two cell types are highly interconnected during cerebellar development through the production of molecules that help their proliferation, differentiation and maintenance. In this article, we present data showing that OL ablation has major effects on the physiology of Purkinje (PC) and granule cells (GC). In particular, OL ablation results into a reduction of sonic hedgehog (Shh), Brain Derived Neurotrophic Factor (BDNF), and Reelin (Rln) expression. These results indicate that absence of OLs profoundly alters the normal cerebellar developmental program.

• OSTI ID: 20972096
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 13 Vol. 313; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ABLATION
CEREBELLUM
IN VIVO
MYELIN
NERVE CELLS
PHENOTYPE
PHYSIOLOGY
TRANSGENIC MICE