Investigation of the alkaline hydrogen peroxide pretreatment: From cellulose saccharification to lignin isolation

Zhan, Yunni; Liu, Xuze; Huang, Chen; Zhou, Xuelian; Lyu, Yan; Lin, Yan; Huang, Caoxing; Ma, Wencan; Xie, Zhanghong; Fang, Guigan; Ragauskas, Arthur J.

doi:10.1016/j.indcrop.2024.118533

Investigation of the alkaline hydrogen peroxide pretreatment: From cellulose saccharification to lignin isolation

Journal Article · Thu Apr 18 00:00:00 EDT 2024 · Industrial Crops and Products

DOI:https://doi.org/10.1016/j.indcrop.2024.118533· OSTI ID:2406724

Zhan, Yunni ^[1]; Liu, Xuze ^[1]; Huang, Chen ^[2]; Zhou, Xuelian ^[1]; Lyu, Yan ^[1]; Lin, Yan ^[1]; Huang, Caoxing ^[3]; Ma, Wencan ^[1]; Xie, Zhanghong ^[4]; Fang, Guigan ^[2]; ^[5]

Chinese Academy of Forestry (China)
Chinese Academy of Forestry (China); Nanjing Forestry Univ. (China)
Nanjing Forestry Univ. (China)
Yibin Paper Industry Co., Ltd. (China)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS)

Alkaline hydrogen peroxide (AHP) pretreatment, a green and effective technology for biomass fractionation, was studied to overcome the recalcitrance of bamboo for lignin isolation and facilitating enzymatic saccharification. In order to explore the impact of AHP pretreatment on the cellulose saccharification of bamboo, the glucan/xylan recovery, lignin removal, and structural properties of solid substrate were investigated in this research. AHP pretreatment at 90 °C for 60 min with 3.0 % H₂O₂ resulted in the maximum glucan/xylan hydrolysis yield reaching 90.62 % and 88.30 %, respectively. Specially, for the first time, the recovered lignin from the AHP liquid possesses up to 59.84/100Ar of β-O-4 linkages, abundant ferulic acid (FA), and p-coumarate (pCE) structures, as well as a homogeneous molecular structure with high purity (84.04%-96.20%) and preferable antioxidant activities (IC50: 0.015–0.018 mg/mL). Finally, these results provided theoretical support for the comprehensive valorization of lignocellulose, which is favorable for achieving viable biorefineries.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 2406724

Journal Information:: Industrial Crops and Products, Journal Name: Industrial Crops and Products Vol. 214; ISSN 0926-6690

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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