The bamboo delignification saturation point in alkaline hydrogen peroxide pretreatment and its association with enzymatic hydrolysis

Huang, Chen; Zhan, Yunni; Cheng, Jinyuan; Wang, Jia; Meng, Xianzhi; Fang, Guigan; Ragauskas, Arthur J.

doi:10.1016/j.biortech.2022.127462

Title: The bamboo delignification saturation point in alkaline hydrogen peroxide pretreatment and its association with enzymatic hydrolysis

Journal Article · Sat Jun 11 00:00:00 EDT 2022 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2022.127462· OSTI ID:1883823

Huang, Chen ^[1]; Zhan, Yunni ^[1]; Cheng, Jinyuan ^[1]; Wang, Jia ^[2]; Meng, Xianzhi ^[3]; Fang, Guigan ^[1];

^[3]

Chinese Academy of Forestry, Nanjing (China)
Nanjing Forestry Univ. (China)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

A delignification saturation point (DSP) was observed for bamboo alkaline hydrogen peroxide pretreatment (AHP). Lignin removal was increased from 52.23% to ~70% when increasing H₂O₂ dosage from 0% to 2% at the optimum pH, but it cannot be further reinforced as increasing the H₂O₂. With partial lignin preserved, the glucan hydrolysis yield was found to have a ceiling of ~80%. This work indicated a strong association between enzymatic digestibility and lignin removal. Anatomical analysis by fluorescence microscope and confocal Raman microscope revealed that the undegradable lignin was mainly existing in the cell corner of sclerenchyma fibers, causing the DSP in the bamboo AHP. Finally, the residual lignin in pretreated bamboo was characterized with GPC, HSQC NMR, and ³¹P NMR, which revealed the nature of DSP. This study could help to understand the lignin modification during the AHP and further contribute to the establishment of a chemical-saving biorefinery.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE; National Natural Science Foundation for Youth; Jiangsu Province Key Laboratory of Biomass Engineering and Materials; Natural Science Foundation of Jiangsu Province; National Natural Science Foundation of China (NSFC); University of Tennessee

Grant/Contract Number:: AC05-00OR22725; 32001273; JSBEM-S-202004; JSBEM-S-202203; BK2020789; 52006106

OSTI ID:: 1883823

Journal Information:: Bioresource Technology, Vol. 359, Issue 1; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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