Microscale dynamics in thermoreversible hydrogels: Impact of probe size and concentration

Zhu, Hengwei; Quah-Ivarson, Suan P.; Zhang, Yugang; Fluerasu, Andrei; Yu, Xiaoxi; Zheng, Bingqian; Yin, Xuechen; Liu, Weiping; Bhatia, Surita R.

doi:10.1016/j.eurpolymj.2023.112434

Microscale dynamics in thermoreversible hydrogels: Impact of probe size and concentration

Journal Article · Thu Sep 21 00:00:00 EDT 2023 · European Polymer Journal

DOI:https://doi.org/10.1016/j.eurpolymj.2023.112434· OSTI ID:2403447

Zhu, Hengwei ^[1]; ^[1]; ^[2]; Fluerasu, Andrei ^[2]; ^[1]; Zheng, Bingqian ^[1]; Yin, Xuechen ^[1]; Liu, Weiping ^[1]; ^[1]

Stony Brook Univ., NY (United States)
Brookhaven National Laboratory (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II)

Passive microrheology techniques using dynamic light scattering (DLS) and X-ray photon correlation spectroscopy (XPCS) have emerged as important techniques for characterizing the dynamics and viscoelastic properties of soft polymeric biomaterials. However, the impact of probe particle type, size, and concentration are important considerations in interpretation of results and comparison to properties obtained from bulk rheology measurements. In this work, we investigate a model thermoreversible polymeric hydrogel and compare results from DLS-microrheology with different size and concentrations of polystyrene probe particles and XPCS-microrheology with inorganic nanoparticles. We obtained trends that aligned most closely with the macroscopic rheology with probe particles that are slightly larger than the characteristic length scale of the gel structure, but within the same order of magnitude. By contrast, larger probe particles yielded microrheology data that are more dominated by elastic behavior than what we might expect from bulk rheology experiments, while use of small inorganic nanoparticles in XPCS-microrheology resulted in a more a more viscous response than would be expected based on the bulk rheology. Finally, this study demonstrates the utility of passive DLS- and XPCS-based microrheology in characterizing the rheological properties of complex polymeric biomaterials, while also highlighting important considerations in experimental design and choice of type, size, and concentration of probe particles used.

View Accepted Manuscript (DOE)

Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); National Science Foundation (NSF)

Grant/Contract Number:: SC0012704

OSTI ID:: 2403447

Report Number(s):: BNL--225816-2024-JAAM

Journal Information:: European Polymer Journal, Journal Name: European Polymer Journal Vol. 199; ISSN 0014-3057

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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