Evaluating Ion Accumulation and Storage in Traveling Wave Based Structures for Lossless Ion Manipulations

Kwantwi-Barima, Pearl; Garimella, Sandilya B.; Attah, Isaac K.; Ibrahim, Yehia M.

doi:10.1021/jasms.3c00348

Evaluating Ion Accumulation and Storage in Traveling Wave Based Structures for Lossless Ion Manipulations

Journal Article · Mon Nov 20 00:00:00 EST 2023 · Journal of the American Society for Mass Spectrometry

DOI:https://doi.org/10.1021/jasms.3c00348· OSTI ID:2340832

Kwantwi-Barima, Pearl ^[1]; ^[1]; ^[1]; ^[1]

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Structures for lossless ion manipulations (SLIM) technology has demonstrated high resolving power ion mobility separation and flexibility to integrate complex ion manipulations into a single experimental platform. To enable IMS separations, trapping/accumulating ions inside SLIM (or in-SLIM) prior to injection of a packet for separations provides ease of operation and reduces the need for dedicated ion traps external to SLIM. To fully characterize the ion accumulation process, we have evaluated the effect of TW amplitudes, ion collection times, and storage times on the “in-SLIM” accumulation process. The study utilized a SLIM module comprising 5 distinct tracks, each with a specific ion accumulation configuration. The effect of the TW conditions on the accumulation process was investigated for a 3-peptide mixture: kemptide, angiotensin II, and neurotensin at a TW speed of 106 m/s. The effect of ion accumulation time/collection time and storage time was investigated, in addition to TW amplitude. Overall, the signal of the analyte ions increased when the ion collection time increased from 49 to 163 ms but decreased when the ion collection time increased further to 652 ms due to the space charge effects. Ion losses were observed at high TW amplitudes (e.g., 15 V_p–p and 20 V_p–p). In addition, under space charge conditions (e.g., collection times of 163 and 652 ms), the signal of the analyte ions decreased with an increase in storage times for all TW amplitudes applied to the trapping region. For ion accumulation, the data indicate that gentler TW conditions must be utilized to minimize ion losses and fragments to benefit from the “in-SLIM” accumulation process. Finally, wider SLIM tracks provided better performance than those with narrower tracks.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 2340832

Report Number(s):: PNNL-SA--190819

Journal Information:: Journal of the American Society for Mass Spectrometry, Journal Name: Journal of the American Society for Mass Spectrometry Journal Issue: 12 Vol. 34; ISSN 1044-0305

Publisher:: American Society for Mass SpectrometryCopyright Statement

Country of Publication:: United States

Language:: English

References (34)

Applications of a travelling wave-based radio-frequency-only stacked ring ion guide Giles, Kevin; Pringle, Steven D.; Worthington, Kenneth R. Rapid Communications in Mass Spectrometry, Vol. 18, Issue 20, p. 2401-2414 https://doi.org/10.1002/rcm.1641	journal	October 2004
Ion Mobility Separations of Isomers based upon Long Path Length Structures for Lossless Ion Manipulations Combined with Mass Spectrometry Deng, Liulin; Ibrahim, Yehia M.; Baker, Erin S. ChemistrySelect, Vol. 1, Issue 10 https://doi.org/10.1002/slct.201600460	journal	July 2016
Ultra-high-resolution ion mobility spectrometry—current instrumentation, limitations, and future developments Kirk, Ansgar T.; Bohnhorst, Alexander; Raddatz, Christian-Robert Analytical and Bioanalytical Chemistry, Vol. 411, Issue 24 https://doi.org/10.1007/s00216-019-01807-0	journal	April 2019
Detecting and Removing Data Artifacts in Hadamard Transform Ion Mobility-Mass Spectrometry Measurements Prost, Spencer A.; Crowell, Kevin L.; Baker, Erin S. Journal of The American Society for Mass Spectrometry, Vol. 25, Issue 12 https://doi.org/10.1007/s13361-014-0895-y	journal	May 2014
Cyclic Ion Mobility Mass Spectrometry Distinguishes Anomers and Open-Ring Forms of Pentasaccharides Ujma, Jakub; Ropartz, David; Giles, Kevin Journal of The American Society for Mass Spectrometry, Vol. 30, Issue 6 https://doi.org/10.1007/s13361-019-02168-9	journal	April 2019
Evaluating dynamic traveling wave profiles for the enhancement of separation and sensitivity in traveling wave structures for lossless ion manipulations Kinlein, Zackary; Clowers, Brian H. Journal of Chromatography A, Vol. 1706 https://doi.org/10.1016/j.chroma.2023.464207	journal	September 2023
Accelerating prototyping experiments for traveling wave structures for lossless ion manipulations Kinlein, Zackary R.; Anderson, Gordon A.; Clowers, Brian H. Talanta, Vol. 244 https://doi.org/10.1016/j.talanta.2022.123446	journal	July 2022
A Split-Field Drift Tube for Separation and Efficient Fragmentation of Biomolecular Ions Valentine, Stephen J.; Koeniger, Stormy L.; Clemmer, David E. Analytical Chemistry, Vol. 75, Issue 22 https://doi.org/10.1021/ac030111r	journal	November 2003
Hadamard Transform Ion Mobility Spectrometry Clowers, Brian H.; Siems, William F.; Hill, Herbert H. Analytical Chemistry, Vol. 78, Issue 1 https://doi.org/10.1021/ac050615k	journal	January 2006
Multiplexed Ion Mobility Spectrometry-Orthogonal Time-of-Flight Mass Spectrometry Belov, Mikhail E.; Buschbach, Michael A.; Prior, David C. Analytical Chemistry, Vol. 79, Issue 6, p. 2451-2462 https://doi.org/10.1021/ac0617316	journal	March 2007
Ion Funnel Trap Interface for Orthogonal Time-of-Flight Mass Spectrometry Ibrahim, Yehia; Belov, Mikhail E.; Tolmachev, Aleksey V. Analytical Chemistry, Vol. 79, Issue 20 https://doi.org/10.1021/ac071091m	journal	October 2007
High Resolution Trapped Ion Mobility Spectrometery of Peptides Silveira, Joshua A.; Ridgeway, Mark E.; Park, Melvin A. Analytical Chemistry, Vol. 86, Issue 12 https://doi.org/10.1021/ac501261h	journal	May 2014
Enhanced Ion Utilization Efficiency Using an Electrodynamic Ion Funnel Trap as an Injection Mechanism for Ion Mobility Spectrometry Clowers, Brian H.; Ibrahim, Yehia M.; Prior, David C. Analytical Chemistry, Vol. 80, Issue 3, p. 612-623 https://doi.org/10.1021/ac701648p	journal	February 2008
Dynamically Multiplexed Ion Mobility Time-of-Flight Mass Spectrometry Belov, Mikhail E.; Clowers, Brian H.; Prior, David C. Analytical Chemistry, Vol. 80, Issue 15 https://doi.org/10.1021/ac8003665	journal	August 2008
An Ion Trap Interface for ESI−Ion Mobility Experiments Hoaglund, Cherokee S.; Valentine, Stephen J.; Clemmer, David E. Analytical Chemistry, Vol. 69, Issue 20 https://doi.org/10.1021/ac970526a	journal	October 1997
U-Shaped Mobility Analyzer: A Compact and High-Resolution Counter-Flow Ion Mobility Spectrometer Wang, Keke; Qiu, Ran; Zhang, Xiaoqiang Analytical Chemistry, Vol. 92, Issue 12 https://doi.org/10.1021/acs.analchem.0c00868	journal	May 2020
Ultra-High-Resolution Ion Mobility Separations Over Extended Path Lengths and Mobility Ranges Achieved using a Multilevel Structures for Lossless Ion Manipulations Module Hollerbach, Adam L.; Li, Ailin; Prabhakaran, Aneesh Analytical Chemistry, Vol. 92, Issue 11 https://doi.org/10.1021/acs.analchem.0c01397	journal	May 2020
Masked Multiplexed Separations to Enhance Duty Cycle for Structures for Lossless Ion Manipulations Clowers, Brian H.; Cabrera, Elvin; Anderson, Gordon Analytical Chemistry, Vol. 93, Issue 14 https://doi.org/10.1021/acs.analchem.0c04799	journal	April 2021
A Miniature Multilevel Structures for Lossless Ion Manipulations Ion Mobility Spectrometer with Wide Mobility Range Separation Capabilities Hollerbach, Adam L.; Norheim, Randolph V.; Kwantwi-Barima, Pearl Analytical Chemistry, Vol. 94, Issue 4 https://doi.org/10.1021/acs.analchem.1c04700	journal	December 2021
Development of a Structure for Lossless Ion Manipulations (SLIM) High Charge Capacity Array of Traps Huntley, Adam P.; Hollerbach, Adam L.; Prabhakaran, Aneesh Analytical Chemistry, Vol. 95, Issue 9 https://doi.org/10.1021/acs.analchem.2c05025	journal	February 2023
Ion Trapping, Storage, and Ejection in Structures for Lossless Ion Manipulations Zhang, Xinyu; Garimella, Sandilya V. B.; Prost, Spencer A. Analytical Chemistry, Vol. 87, Issue 12, p. 6010-6016 https://doi.org/10.1021/acs.analchem.5b00214	journal	May 2015
Ultra-High Resolution Ion Mobility Separations Utilizing Traveling Waves in a 13 m Serpentine Path Length Structures for Lossless Ion Manipulations Module Deng, Liulin; Ibrahim, Yehia M.; Hamid, Ahmed M. Analytical Chemistry, Vol. 88, Issue 18 https://doi.org/10.1021/acs.analchem.6b01915	journal	August 2016
Analysis of Native-Like Ions Using Structures for Lossless Ion Manipulations Allen, Samuel J.; Eaton, Rachel M.; Bush, Matthew F. Analytical Chemistry, Vol. 88, Issue 18 https://doi.org/10.1021/acs.analchem.6b02089	journal	September 2016
Greatly Increasing Trapped Ion Populations for Mobility Separations Using Traveling Waves in Structures for Lossless Ion Manipulations Deng, Liulin; Ibrahim, Yehia M.; Garimella, Sandilya V. B. Analytical Chemistry, Vol. 88, Issue 20 https://doi.org/10.1021/acs.analchem.6b02678	journal	September 2016
Development of an Ion Mobility Spectrometry-Orbitrap Mass Spectrometer Platform Ibrahim, Yehia M.; Garimella, Sandilya V. B.; Prost, Spencer A. Analytical Chemistry, Vol. 88, Issue 24 https://doi.org/10.1021/acs.analchem.6b03027	journal	December 2016
Serpentine Ultralong Path with Extended Routing (SUPER) High Resolution Traveling Wave Ion Mobility-MS using Structures for Lossless Ion Manipulations Deng, Liulin; Webb, Ian K.; Garimella, Sandilya V. B. Analytical Chemistry, Vol. 89, Issue 8 https://doi.org/10.1021/acs.analchem.7b00185	journal	April 2017
Correlating Resolving Power, Resolution, and Collision Cross Section: Unifying Cross-Platform Assessment of Separation Efficiency in Ion Mobility Spectrometry Dodds, James N.; May, Jody C.; McLean, John A. Analytical Chemistry, Vol. 89, Issue 22 https://doi.org/10.1021/acs.analchem.7b02827	journal	October 2017
Combining Ultrahigh-Resolution Ion-Mobility Spectrometry with Cryogenic Infrared Spectroscopy for the Analysis of Glycan Mixtures Ben Faleh, Ahmed; Warnke, Stephan; Rizzo, Thomas R. Analytical Chemistry, Vol. 91, Issue 7 https://doi.org/10.1021/acs.analchem.9b00659	journal	February 2019
Resolving Power and Collision Cross Section Measurement Accuracy of a Prototype High-Resolution Ion Mobility Platform Incorporating Structures for Lossless Ion Manipulation May, Jody C.; Leaptrot, Katrina L.; Rose, Bailey S. Journal of the American Society for Mass Spectrometry, Vol. 32, Issue 4 https://doi.org/10.1021/jasms.1c00056	journal	March 2021
Collision Cross-Section Calibration Strategy for Lipid Measurements in SLIM-Based High-Resolution Ion Mobility Rose, Bailey S.; May, Jody C.; Reardon, Allison R. Journal of the American Society for Mass Spectrometry, Vol. 33, Issue 7 https://doi.org/10.1021/jasms.2c00067	journal	June 2022
A Flexible, Modular Platform for Multidimensional Ion Mobility of Native-like Ions Eaton, Rachel M.; Zercher, Benjamin P.; Wageman, AnneClaire Journal of the American Society for Mass Spectrometry, Vol. 34, Issue 6 https://doi.org/10.1021/jasms.3c00112	journal	May 2023
A two-phase approach to fourier transform ion mobility time-of-flight mass spectrometry Clowers, Brian H.; Siems, William F.; Yu, Zhihao The Analyst, Vol. 140, Issue 20 https://doi.org/10.1039/C5AN00941C	journal	January 2015
New frontiers for mass spectrometry based upon structures for lossless ion manipulations Ibrahim, Yehia M.; Hamid, Ahmed M.; Deng, Liulin The Analyst, Vol. 142, Issue 7 https://doi.org/10.1039/C7AN00031F	journal	January 2017
Toward artifact-free data in Hadamard transform-based double multiplexing of ion mobility-Orbitrap mass spectrometry Tummalacherla, Meghasyam; Garimella, Sandilya V. B.; Prost, Spencer A. The Analyst, Vol. 142, Issue 10 https://doi.org/10.1039/C7AN00289K	journal	January 2017

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