Identification and characterization of human bone-derived cells

Journal Article · Mon Jan 15 04:00:00 EST 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2017.11.155· OSTI ID:23127526

Jo, Sungsin ^[1]; Lee, Jin Kyu ^[2]; Han, Jinil ^[3]; Lee, Bitnara; Kang, Suman ^[1]; Hwang, Kyu-Tae ^[2]; Park, Ye-Soo ^[4]; Kim, Tae-Hwan ^[1]

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1. Hanyang University Hospital for Rheumatic Diseases, Seoul (Korea, Republic of)
2. Department of Orthopedic Surgery, Hanyang University Hospital, Seoul (Korea, Republic of)
3. Gencurix, Inc, Hanhwan Bizmetro 1, Guro 3-dong, Guro-gu, Seoul (Korea, Republic of)
4. Department of Orthopedic Surgery, Hanyang University Hospital, Guri (Korea, Republic of)

Highlights: • In non-osteogenic condition, BdCs are more correlated to MSCs than FOBs in bioinformatics result. • BdCs sustained not only high ALP, OPN and osterix expression, but differentiated to mature osteoblast. • In osteogenic condition, BdCs are similar with FOBs than MSCs in physiological changes results. • We suggest BdCs are osteoprogenitor cells or undifferentiated preosteoblasts. This study was designed to identify and characterize primary bone-derived cells (BdCs) and investigate the potential role of osteoblast differentiation. Primary BdCs were isolated from surgical bone for comparative analysis with mesenchymal stem cells (MSCs) and fetal osteoblasts (FOBs) and for potential differentiation to mature osteoblasts. Using three different cells, we successfully cultivated human osteoblast differentiation and activity which were evaluated using microarray and biochemical methods. BdCs are more correlated to MSCs in bioinformatics result and similar with FOBs in gene expression. In particular, Osterix, osteoprogenitor marker, was high expressed in BdCs, while the expression in MSCs and FOBs were very low. Furthermore, BdCs exhibited a marked alkaline phosphatase (ALP) expression, early stage of osteogenic marker, and retained osteogenic properties and physiological changes into maturation as in FOBs. BdCs also showed an increase in bone morphogenic protein 2 (BMP2), osteopontin (OPN), and osteocalcin (OCN) mRNA expressions during differentiation. This study suggests that BdCs may be osteoprogenitor cells or undifferentiated preosteoblasts with strong capacity to differentiate toward mature osteoblasts.

• OSTI ID: 23127526
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 495; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ALKALINE PHOSPHATASE
CONNECTIVE TISSUE CELLS
MESSENGER-RNA
SKELETON
STEM CELLS
SURGERY