Hsa{sub c}ircRNA{sub 1}03809 regulated the cell proliferation and migration in colorectal cancer via miR-532–3p / FOXO4 axis
- Department of General Surgery, The Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226000 (China)
- Department of Emergency Surgery, The Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226000 (China)
- Department of Otorhinolaryngology, The Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226000 (China)
- Department of Endocrinology, The Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226000 (China)
Highlights: • The expression of hsa{sub c}ircRNA{sub 1}03809 is down-regulated in the CRC tissues and cells. • Knockdown of hsa{sub c}ircRNA{sub 1}03809 promotes cell proliferation in CRC. • Knockdown of hsa{sub c}ircRNA{sub 1}03809 promotes cell migration in CRC. • Hsa{sub c}ircRNA{sub 1}03809 acts as a suppressor via regulation of miR-532–3P/FOXO4 axis in CRC. Circular RNAs(circRNAs) are a class of non-coding RNAs that are widely expressed in a variety of cell species. The role they play in cancers is poorly understood, especially in colorectal cancer (CRC). Hsa{sub c}ircRNA{sub 1}03809 (hsa{sub c}irc{sub 0}072088, circZFR)has been demonstrated to be lowly expressed in colorectal cancer tissues and is associated with stage and lymph node metastasis of cancer tissues. Real-time quantitative PCR (qRT-PCR) was used to verify the relationship of hsa{sub c}ircRNA{sub 1}03809 between colorectal cancer and paired adjacent tissue in clinical tissue samples. Then, the proliferative capacity, migration ability, cell cycle, and apoptosis were measured using wound-healing assay, CCK8, transwell assay, flow cytometry, and the like, when hsa{sub c}ircRNA{sub 1}03809 expression in SW620 and COCA-2. The qRT-PCR, western bolt and other experiments verify that the expression of hsa{sub c}ircRNA{sub 1}03809 can regulate the expression of miR-532–3P and FOXO4. Hsa{sub c}ircRNA{sub 1}03809 was found to be significantly down regulated in CRC tissues and cell lines and compared with paired adjacent non-tumorous tissues and normal FHC cells. Hsa{sub c}ircRNA{sub 1}03809 participates in the regulation of biological functions through the miR-532–3P/FOXO4 axis in the CRC. Hsa{sub c}ircRNA{sub 1}03809 may be a potential novel gene target for the diagnosis and treatment of CRC.
- OSTI ID:
- 23107814
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 505, Issue 2; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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