A novel circular RNA, hsa{sub c}irc{sub 0}046701, promotes carcinogenesis by increasing the expression of miR-142-3p target ITGB8 in glioma

Journal Article · Thu Mar 15 00:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.01.076· OSTI ID:23137256

Li, Guobin; Yang, Haiying; Han, Kun ^[1]; Zhu, Dezhang ^[2]; Lun, Peng; Zhao, Yan ^[1]

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1. Department of Neurosurgery, The Affiliated Hospital of Qingdao University (China)
2. Department of Anesthesiology, The Affiliated Hospital of Qingdao University (China)

Highlights: • We identified a novel circular RNA, hsa{sub c}irc{sub 0}046701 in glioma cells. • Hsa{sub c}irc{sub 0}046701 was significantly upregulated in glioma tissues. • Hsa{sub c}irc{sub 0}046701 sponges miR-142-3p in glioma. • MiR-142-3p directly targets ITGB8 in glioma. • Hsa{sub c}irc{sub 0}046701/miR-142-3p/ITGB8 axis plays critical roles in glioma pathogenesis. In the present study, we identified a novel circular RNA (circRNA), hsa{sub c}irc{sub 0}046701, in glioma cells. We measured the expression of hsa{sub c}irc{sub 0}046701 using qRT-PCR in glioma tissues and cell lines, and explored its functions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation, and Transwell assays. Luciferase reporter assays were performed to validate the correlation between microRNA miR-142-3p and hsa{sub c}irc{sub 0}046701 or integrin subunit beta 8 (ITGB8). The results showed that hsa{sub c}irc{sub 0}046701 was significantly upregulated in glioma tissues and cell lines, and knockdown of hsa{sub c}irc{sub 0}046701 inhibited cell proliferation and invasion. Luciferase reporter assays indicated that hsa{sub c}irc{sub 0}046701 functions as a sponge for miR-142-3p and regulates the expression of ITGB8. Subsequently, functional assays revealed that silencing of hsa{sub c}irc{sub 0}046701 could upregulate miR-142-3p, resulting in downregulation of ITGB8. The results demonstrated that the hsa{sub c}irc{sub 0}046701/miR-142-3p/ITGB8 axis might play critical regulatory roles in the pathogenesis and development of glioma.

• OSTI ID: 23137256
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 498, Issue 1; Other Information: Copyright (c) 2018 Published by Elsevier Inc.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BROMIDES
CARCINOGENESIS
CELL PROLIFERATION
COLONY FORMATION
GLIOMAS
LUCIFERASE
PORIFERA
RNA