Translin modulates mesenchymal cell proliferation and differentiation in mice

Journal Article · Sat Sep 15 04:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.08.141· OSTI ID:23103538

Ikeuchi, Yukiko; Imanishi, Azusa ^[1]; Sudo, Katsuko ^[2]; Fukunaga, Takako; Yokoi, Aya ^[1]; others, and

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1. Division of Medical Biophysics, Kobe University Graduate School of Health Sciences, Kobe, 654-0142 (Japan)
2. Pre-clinical Research Center, Tokyo Medical University, Tokyo, 160-8402 (Japan)

Highlights: • Translin restricts the size of bone marrow mesenchymal stem cells. • Translin impedes the growth of early-stage mesenchymal cells. • Translin inhibits differentiation of bone marrow and adipose mesenchymal cells. • In vitro-fertilized Translin-null mice grow normally and show normal metabolism. Translin, a highly conserved DNA/RNA binding protein that forms a hetero-octamer together with Translin-associated factor X (TRAX), possesses a broad variety of functions, including RNA processing and DNA repair. Recent studies have reported that Translin is involved in mesenchymal cell physiology. Thus, here we analyzed the intrinsic role of Translin in mesenchymal cell proliferation and differentiation. Translin-deficient E11.5 mouse embryonic fibroblasts showed enhanced growth. Translin-deficient bone marrow-derived mesenchymal stem cells showed substantial expansion in vivo and enhanced proliferation in vitro. These cells also showed enhanced osteogenic and adipocytic differentiation. Histological analyses showed adipocytic hypertrophy in various adipose tissues. Translin knockout did not affect the growth of subcutaneous white adipose tissue-derived stem cells, but enhanced adipocytic differentiation was observed in vitro. Contrary to previous reports, in vitro-fertilized Translin-null mice were not runted and exhibited normal metabolic homeostasis, indicating the fragility of these mice to environmental conditions. Together, these data suggest that Translin plays an intrinsic role in restricting mesenchymal cell proliferation and differentiation.

• OSTI ID: 23103538
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 504; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADIPOSE TISSUE
BONE MARROW
CELL DIFFERENTIATION
CELL PROLIFERATION
DNA REPAIR
FIBROBLASTS
MICE
RNA PROCESSING
STEM CELLS