Identification and characterization of UDP-mannose in human cell lines and mouse organs: Differential distribution across brain regions and organs
Journal Article
·
· Biochemical and Biophysical Research Communications
- Department of Academic Research Support Promotion Facility, Center for Research Promotion and Support, Fujita Health University, Toyoake, Aichi 470-1192 (Japan)
- Disease Glycomics Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center, RIKEN Global Research Cluster, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
- Structural Glycobiology Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center, RIKEN Global Research Cluster, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
- Molecular Membrane Neuroscience, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
- Department of Nephrology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192 (Japan)
Highlights: • UDP-Man was detected in mammals for the first time using LC-MS analysis. • UDP-Man was found in cell lines by monitoring characteristic fragment ions. • Cellular UDP-Man levels are strongly elevated in a mannose dose-dependent manner. • Hypothalamus and neocortex contain more UDP-Man than other regions and organs. • UDP-Man likely regulates mannosylation in specific organs or conditions. Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals. Liquid chromatography-mass spectrometry (LC-MS) analyses showed that mouse brain, especially hypothalamus and neocortex, contains higher concentrations of UDP-Man compared to other organs. In cultured human cell lines, addition of mannose in media increased UDP-Man concentrations in a dose-dependent manner. These findings indicate that in mammals the minor nucleotide sugar UDP-Man regulates glycosylation, especially mannosylation in specific organs or conditions.
- OSTI ID:
- 23100628
- Journal Information:
- Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 495; ISSN BBRCA9; ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Plasmodium falciparum dolichol phosphate mannose synthase represents a novel clade
Tetrahymena pyriformis cells are deficient in all mannose-P-dolichol-dependent mannosyltransferases but not in mannose-P-dolichol synthesis
Parallel increase of dolichol pathway and nucleotide pyrophosphatases in rat hepatocytes by dexamethasone
Journal Article
·
Fri Jun 06 00:00:00 EDT 2008
· Biochemical and Biophysical Research Communications
·
OSTI ID:21143708
Tetrahymena pyriformis cells are deficient in all mannose-P-dolichol-dependent mannosyltransferases but not in mannose-P-dolichol synthesis
Journal Article
·
Tue Sep 08 00:00:00 EDT 1987
· Biochemistry; (United States)
·
OSTI ID:5879371
Parallel increase of dolichol pathway and nucleotide pyrophosphatases in rat hepatocytes by dexamethasone
Conference
·
Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:7246479