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Tetrahymena pyriformis cells are deficient in all mannose-P-dolichol-dependent mannosyltransferases but not in mannose-P-dolichol synthesis

Journal Article · · Biochemistry; (United States)
DOI:https://doi.org/10.1021/bi00392a054· OSTI ID:5879371
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Cells of the ciliated protozoan Tetrahymena pyriformis incubated with (/sup 14/C)glucose were found to synthesize Man-P-dolichol and Glc-P-dolichol, as well as Glc/sub 3/Man/sub 5/GlcNAc/sub 2/-P-P-dolichol, the latter being the main and largest lipid derivative formed. The missing mannose residues were those known to be transferred from Man-P-dolichol in other systems. Formation of Man-P-dolichol and of dolichol-P-P-oligosaccharides containing up to five mannose units was detected in cell-free assays containing protozoan membranes, rat liver dolichol-P, unlabeled Man/sub 4-9/GlcNAc/sub 2/-P-P-dolichol from pig liver, and GDP-(/sup 14/C)Man. Under exactly the same conditions but with UDP-(/sup 14/C)Glc instead of GDP-(/sup 14/C)Man, Glc-P-dolichol and dolichol-P-P-oligosaccharides containing five mannose and one to three glucose residues were formed in the absence of the pig liver compounds. In the presence of the latter, dolichol-P-P derivatives containing nine mannose and one to three glucose units were also synthesized. It is concluded that T. pyriformis cells are deficient in all Man-P-dolichol-dependent mannosyltransferases but not in Man-P-dolichol synthesis. The role of the latter compound in this microorganism is unknown.
Research Organization:
Instituto de Investigaciones Bioquimicas Fundacion Campomar, Buenos Aires, Argentina
OSTI ID:
5879371
Journal Information:
Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:18; ISSN BICHA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
BIOCHEMISTRY
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMISTRY
CILIATA
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ENZYMES
GLANDS
GLUCOSE
GLYCOSYL TRANSFERASES
HEXOSES
HEXOSYL TRANSFERASES
IN VITRO
INVERTEBRATES
LABELLED COMPOUNDS
LIVER
MAMMALS
MANNOSE
MEMBRANES
MICROORGANISMS
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PROTOZOA
RATS
RODENTS
SACCHARIDES
SWINE
SYNTHESIS
TETRAHYMENA
TRANSFERASES
VERTEBRATES