Scleroglucan Production by Sclerotium rolfsii ATCC 201126 from Amylaceous and Sugarcane Molasses-Based Media: Promising Insights for Sustainable and Ecofriendly Scaling-Up

Journal Article · Sun Dec 15 04:00:00 EST 2019 · Journal of Polymers and the Environment

DOI:https://doi.org/10.1007/S10924-019-01546-4· OSTI ID:22959271

Valdez, Alejandra Leonor ^[1]; Babot, Jaime Daniel ^[2]; Schmid, Jochen ^[3]; Delgado, Osvaldo Daniel

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1. Planta Piloto de Procesos Industriales Microbiológicos, PROIMI-CONICET, Mycodiversity & Mycoprospection Lab (Argentina)
2. Centro de Referencia para Lactobacilos, CERELA-CONICET (Argentina)
3. Norwegian University of Science and Technology, Department of Biotechnology and Food Science (Norway)

Scleroglucan is a β-glucan exopolysaccharide (EPS) efficiently produced by Sclerotium rolfsii ATCC 201126, with attractive properties for a wide range of industries. Its production was herein comparatively assessed with nine alternative C- and twelve N-sources. When comparing conventional sucrose-based Production Medium PM{sub 20} (8.41 g C/L + NaNO{sub 3} as N-source) at shake-flask-scale vs. alternative C-source versions, soluble starch and sugarcane molasses led to efficient EPS production. At bioreactor scale, starch-based medium led to highest EPS production (7.95 g/L), recovery (~ 52%) and productivities (0.11 g EPS/L h; 0.018 g EPS/g{sub biomass} h). Molasses, though leading to lower EPS production (5.11 g/L), could be envisaged as a promising agroindustrial sub-product for adding value and innovation. Oxalate side-product varied with C- and N-sources, with no clear detrimental relationship with EPS production. Agroindustrial sub-products showed then to be suitable as alternative substrates for efficient, low-cost, and scalable EPS production, thus opening new perspectives for medium reformulation and sustainable EPS production.

• OSTI ID: 22959271
• Journal Information: Journal of Polymers and the Environment, Journal Name: Journal of Polymers and the Environment Journal Issue: 12 Vol. 27; ISSN 1566-2543
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
AGRICULTURE
BIOREACTORS
FOURIER TRANSFORM SPECTROMETERS
FUNGI
INFRARED SPECTRA
MOLASSES
OXALATES
POLYSACCHARIDES
SACCHAROSE
SODIUM NITRATES
SPECTROPHOTOMETERS
SUGAR CANE