Ectopic Organogenesis after Allotransplantation of Freshly Removed or Cryopreserved Neonatal Testicle under the Renal Capsule in Rats
Journal Article
·
· Bulletin of Experimental Biology and Medicine
- N. A. Lopatkin Research Institute of Urology and Interventional Radiology, Affiliated Branch of National Medical Research Radiological Center, Ministry of Health of the Russian Federation (Russian Federation)
- Russian Academy of Sciences, All-Russian Institute of Scientific and Technical Information (Russian Federation)
In experiments on white outbred male rats, a freshly removed (20 experiments) or cryopreserved (10 experiments) testicle from newborn rats (1-2 days after birth) was transplanted under the renal capsule after bilateral orchiectomy. In all experiments with transplantation of freshly removed testicle, it was engrafted. In 3 months, histological examination revealed the formation of mature seminiferous tubules, but spermatogenesis was blocked at the stage of spermatogonia; groups of proliferating Leydig cells in the loose connective tissue between the tubules were also seen. In 6 and 12 months, the status of the seminiferous tubules remained unchanged, but structures typical of the epididymis and developing vas deferens were revealed. The number of proliferating Leydig cells increased. The initially low testosterone concentration in the blood of castrated males increased significantly as soon as in 1 month after transplantation and grew up to 3 months, remaining at a level ~50% of normal. Engraftment of cryopreserved neonatal testicular tissue was observed in 60% cases, however, engrafted tissue, similar to the fresh one, retained the ability for organogenesis with the formation of mature seminiferous tubules, epididymis, and groups of proliferating Leydig cells. The dynamics of blood testosterone concentration in rats with cryopreserved and fresh transplantation was similar. Subcapsular transplantation did not adversely affect the kidneys, which was seen from normal histological structure of the kidneys and creatinine and urea concentrations in the blood.
- OSTI ID:
- 22927940
- Journal Information:
- Bulletin of Experimental Biology and Medicine, Journal Name: Bulletin of Experimental Biology and Medicine Journal Issue: 2 Vol. 166; ISSN BEXBAN; ISSN 0007-4888
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cadmium inhibits testis and epididymal acidification in vivo
EXPERIMENTAL STUDIES ON THE NATURE OF RADIATION HAZARDS. II. INFLUENCES OF CYTOTOXIN, PRODUCED AT THE TIME OF X-RAY IRRADIATION IN A LARGE DOSE, ON THE RECOVERY OF TESTICLE DISTURBANCES
High-fat diet aggravates 2,2′,4,4′-tetrabromodiphenyl ether-inhibited testosterone production via DAX-1 in Leydig cells in rats
Conference
·
Fri Feb 28 23:00:00 EST 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:5523991
EXPERIMENTAL STUDIES ON THE NATURE OF RADIATION HAZARDS. II. INFLUENCES OF CYTOTOXIN, PRODUCED AT THE TIME OF X-RAY IRRADIATION IN A LARGE DOSE, ON THE RECOVERY OF TESTICLE DISTURBANCES
Journal Article
·
Thu Dec 31 23:00:00 EST 1959
· Nippon Igaku Hoshasen Gakkai Zasshi (Japan)
·
OSTI ID:4828469
High-fat diet aggravates 2,2′,4,4′-tetrabromodiphenyl ether-inhibited testosterone production via DAX-1 in Leydig cells in rats
Journal Article
·
Mon May 15 00:00:00 EDT 2017
· Toxicology and Applied Pharmacology
·
OSTI ID:22690971