Morphological Changes in the Retina Under Conditions of Experimental In Vivo Regional Ischemia/Reperfusion

Journal Article · Sat Jun 15 04:00:00 EDT 2019 · Bulletin of Experimental Biology and Medicine

DOI:https://doi.org/10.1007/S10517-019-04511-2· OSTI ID:22927653

Chudin, A. V.; Khoroshilova-Maslova, I. P.; Shchipanova, A. I.; Maibogin, A. M.; Zaitsev, M. S. ^[1]

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1. Ministry of Health of the Russian Federation, Helmholtz Moscow Research Institute of Eye Diseases (Russian Federation)

Two models of retinal ischemia/reperfusion were developed in an experiment on rats and structural changes in eye tissues in the early and late postischemic periods were studied. Ischemia/reperfusion was modeled by elevation of intraocular pressure to 110 mm Hg for 30 min with air injection into the anterior chamber of the eye with a special device or subconjunctival administration of 0.2 ml 4×10{sup –6} M endothelin-1. Morphological studies of the retina of enucleated eyes were performed in 3, 7, and 30 days after ischemia/reperfusion. In 3 days, signs of retinal ischemia were seen (retinal edema and ganglion cell damage). In the late post-ischemic period (30 days), atrophy of the outer nuclear and outer plexiform layer of the retina was observed in animals with retinal ischemia/reperfusion caused by intraocular pressure elevation and complete destruction of neuronal cells was found after administration of endothelin-1.

• OSTI ID: 22927653
• Journal Information: Bulletin of Experimental Biology and Medicine, Journal Name: Bulletin of Experimental Biology and Medicine Journal Issue: 2 Vol. 167; ISSN BEXBAN; ISSN 0007-4888
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ATROPHY
EDEMA
ENDOTHELINS
GANGLIONS
IN VIVO
ISCHEMIA
MORPHOLOGICAL CHANGES
RATS
RETINA