Direct Write Assembly of Graphene/Poly(ε-Caprolactone) Composite Scaffolds and Evaluation of Their Biological Performance Using Mouse Bone Marrow Mesenchymal Stem Cells

Journal Article · Thu Aug 15 04:00:00 EDT 2019 · Applied Biochemistry and Biotechnology

DOI:https://doi.org/10.1007/S12010-019-02976-5· OSTI ID:22927638

Scaffold and mesenchymal stem cell–based cartilage tissue engineering offers a favorable way for the repair and regeneration of injured cartilage. In this study, poly (ε-caprolactone) PCL scaffolds with grid-like structure having periodic lattice was manufactured by robocasting method in the presence of graphene nanoplatelets for cartilage tissue engineering applications. For this purpose, a PCL solution (20 wt%) containing pristine graphene nanopowders in the form of platelets was prepared as printing ink and it was dispensed through a nozzle at room temperature to an ethanol bath at 4 °C. The construction of porous scaffolds was made by a layer-by-layer assembly. Results revealed that graphene additions were not detrimental to deposition process and the structure of the resultant scaffolds. In vitro cell tests indicated that the prepared grid-like graphene/PCL composite scaffolds have good cytocompatibility and non-toxicity for mouse bone marrow mesenchymal stem cells. The stem cells attached and proliferated well on the scaffolds and they also demonstrated a chondrogenic differentiation in the absence of transforming growth factors.

• OSTI ID: 22927638
• Journal Information: Applied Biochemistry and Biotechnology, Journal Name: Applied Biochemistry and Biotechnology Journal Issue: 4 Vol. 188; ISSN ABIBDL; ISSN 0273-2289
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BONE MARROW
CARTILAGE
ETHANOL
GRAPHENE
GROWTH FACTORS
IN VITRO
MICE
NANOPOWDERS
NANOSTRUCTURES
STEM CELLS
TOXICITY