Matrigel immobilization on the shish-kebab structured poly(ε-caprolactone) nanofibers for skin tissue engineering

Journal Article · Wed Mar 09 04:00:00 EST 2016 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.4942295· OSTI ID:22589308

Jing, Xin; Mi, Hao-Yang ^[1]; Peng, Xiang-Fang ^[1]; Turng, Lih-Sheng ^[2]

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1. National Engineer Research Center of Novel Equipment for Polymer Processing, The Key Laboratory of Polymer Processing Engineering of Ministry of Education, South China University of Technology, Guangzhou, 510640 (China)
2. Wisconsin Institutes for Discovery, University of Wisconsin-Madison, 53715 (United States)

Surface properties of tissue engineering scaffolds such as topography, hydrophilicity, and functional groups play a vital role in cell adhesion, migration, proliferation, and apoptosis. First, poly(ε-caprolactone) (PCL) shish-kebab scaffolds (PCL-SK), which feature a three-dimensional structure comprised of electrospun PCL nanofibers covered by periodic, self-induced PCL crystal lamellae on the surface, was created to mimic the nanotopography of native collagen fibrils in the extracellular matrix (ECM). Second, matrigel was covalently immobilized on the surface of alkaline hydrolyzed PCL-SK scaffolds to enhance their hydrophilicity. This combined approach not only mimics the nanotopography of native collagen fibrils, but also simulates the surface features of collagen fibrils for cell growth. To investigate the viability of such scaffolds, HEF1 fibroblast cell assays were conducted and the results revealed that the nanotopography of the PCL-SK scaffolds facilitated cell adhesion and proliferation. The matrigel functionalization on PCL-SK scaffolds further enhanced cellular response, which suggested elevated biocompatibility and greater potential for skin tissue engineering applications.

• OSTI ID: 22589308
• Journal Information: AIP Conference Proceedings, Journal Name: AIP Conference Proceedings Journal Issue: 1 Vol. 1713; ISSN 0094-243X; ISSN APCPCS
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
77 NANOSCIENCE AND NANOTECHNOLOGY
ADHESION
ANIMAL TISSUES
APOPTOSIS
CELL PROLIFERATION
COLLAGEN
COVALENCE
CRYSTALS
FIBROBLASTS
LACTONES
LAMELLAE
MATRIX MATERIALS
NANOFIBERS
ORGANIC POLYMERS
SKIN
SURFACE PROPERTIES
SURFACES
THREE-DIMENSIONAL LATTICES