Quantitative proteome analysis of bovine mammary gland reveals protein dynamic changes involved in peak and late lactation stages
Journal Article
·
· Biochemical and Biophysical Research Communications
- National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193 (China)
- Agricultural Commission of Haian County, Jiangsu Province, Haian 226600 (China)
- International Livestock Research Institute Nairobi (Kenya)
Highlights: • Revealed dynamic protein changes between two lactation stages in bovine mammary by TMT-based proteomics (first time). • Longitudinal design avoided the influence of different genetic backgrounds on the DEP detection. • 179 differentially expressed proteins (DEPs) identified. • Identified novel DEPs: AACS, DHCR7, GSTM3, SFRP1 and SFRP4. • Major pathways: PI3K-Akt, localization, lipid metabolic, amino acid transport immune system and apoptotic signaling. Mammary gland is an important organ for milk synthesis and secretion. It undergoes dramatic physiological changes to adapt the shift from peak to late lactation stage. Protein plays a final very vital role in many life functions, and the protein changes during different lactation stages potentially reflect the biology of lactation and the functions of mammary gland in cows. In current study, we adopted tandem mass tags label-based quantitative analysis technique and to investigate proteome changes occurring in bovine mammary gland from peak to late lactation stages. A total of 3753 proteins from mammary tissues taken at two lactation points from four individual cows by biopsy were quantified, out of which 179 proteins were expressed differentially between two stages. We observed five new DEPs (AACS, DHCR7, GSTM3, SFRP1 and SFRP4) and nine functional well-studies known proteins (PLIN2, LPIN1, PLIN3, GSN, CD74, MMP2, SOD1, SOD3 and GPX3) related to milk performance and mammary morphology. Bioinformatics analyses of the DEPs showed a majority of the up-regulated proteins during late lactation stage were related to apoptosis and immune process, while the downregulated proteins were mainly involved in localization, lipid metabolic and transport process. This suggests that the mammary gland can adapt to different molecular functions according to the biological need of the animal. From the integrated analysis of the differentially expressed proteins with known quantitative trait loci and genome-wide association study data, we identified 95 proteins may potentially affect milking performance. We expect findings in this study could be a valuable resource for future studies investigating the bovine proteome and functional studies.
- OSTI ID:
- 22897544
- Journal Information:
- Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1-2 Vol. 494; ISSN 0006-291X; ISSN BBRCA9
- Country of Publication:
- United States
- Language:
- English
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