Reproducible growth of Brachypodium in EcoFAB 2.0 reveals that nitrogen form and starvation modulate root exudation

Novak, Vlastimil; Andeer, Peter F.; Bowen, Benjamin P.; Ding, Yezhang; Zhalnina, Kateryna; Hofmockel, Kirsten S.; Tomaka, Connor; Harwood, Thomas V.; van Winden, Michelle M.; Golini, Amber N.; Kosina, Suzanne M.; Northen, Trent R.

doi:10.1126/sciadv.adg7888

Title: Reproducible growth of Brachypodium in EcoFAB 2.0 reveals that nitrogen form and starvation modulate root exudation

Journal Article · Wed Jan 03 00:00:00 EST 2024 · Science Advances

DOI:https://doi.org/10.1126/sciadv.adg7888· OSTI ID:2337431

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Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Iowa State University, Ames, IA (United States)
Wageningen University & Research (The Netherlands)

Understanding plant-microbe interactions requires examination of root exudation under nutrient stress using standardized and reproducible experimental systems. We grew Brachypodium distachyon hydroponically in fabricated ecosystem devices (EcoFAB 2.0) under three inorganic nitrogen forms (nitrate, ammonium, and ammonium nitrate), followed by nitrogen starvation. Analyses of exudates with liquid chromatography–tandem mass spectrometry, biomass, medium pH, and nitrogen uptake showed EcoFAB 2.0’s low intratreatment data variability. Furthermore, the three inorganic nitrogen forms caused differential exudation, generalized by abundant amino acids–peptides and alkaloids. Comparatively, nitrogen deficiency decreased nitrogen-containing compounds but increased shikimates-phenylpropanoids. Subsequent bioassays with two shikimates-phenylpropanoids (shikimic and p-coumaric acids) on soil bacteria or Brachypodium seedlings revealed their distinct capacity to regulate both bacterial and plant growth. Our results suggest that (i) Brachypodium alters exudation in response to nitrogen status, which can affect rhizobacterial growth, and (ii) EcoFAB 2.0 is a valuable standardized plant research tool.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; AC02-05CH11231; SC0021234

OSTI ID:: 2337431

Alternate ID(s):: OSTI ID: 2281666

Report Number(s):: PNNL-SA-197257

Journal Information:: Science Advances, Vol. 10, Issue 1; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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