N-dependent dynamics of root growth and nitrate and ammonium uptake are altered by the bacterium Herbaspirillum seropedicae in the cereal model Brachypodium distachyon

Kuang, Weiqi; Sanow, Stefan; Kelm, Jana M.; Müller Linow, Mark; Andeer, Peter; Kohlheyer, Dietrich; Northen, Trent; Vogel, John P.; Watt, Michelle; Arsova, Borjana

doi:10.1093/jxb/erac184

N-dependent dynamics of root growth and nitrate and ammonium uptake are altered by the bacterium Herbaspirillum seropedicae in the cereal model Brachypodium distachyon

Journal Article · Thu May 05 00:00:00 EDT 2022 · Journal of Experimental Botany

DOI:https://doi.org/10.1093/jxb/erac184· OSTI ID:1907596

^[1]; ^[2]; Kelm, Jana M. ^[2]; ^[2]; ^[3]; ^[2]; ^[4]; ^[4]; ^[5]; ^[2]

Hunan University of Arts and Science, Changde (China); Chinese Academy of Sciences (CAS), Changsha (China)
Forschungszentrum Jülich (Germany)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)
Forschungszentrum Jülich (Germany); University of Melbourne, VIC (Australia)

Nitrogen (N) fixation in cereals by root-associated bacteria is a promising solution for reducing use of chemical N fertilizers in agriculture. However, plant and bacterial responses are unpredictable across environments. We hypothesized that cereal responses to N-fixing bacteria are dynamic, depending on N supply and time. To quantify the dynamics, a gnotobiotic, fabricated ecosystem (EcoFAB) was adapted to analyse N mass balance, to image shoot and root growth, and to measure gene expression of Brachypodium distachyon inoculated with the N-fixing bacterium Herbaspirillum seropedicae. Phenotyping throughput of EcoFAB-N was 25–30 plants h^-1 with open software and imaging systems. Herbaspirillum seropedicae inoculation of B. distachyon shifted root and shoot growth, nitrate versus ammonium uptake, and gene expression with time; directions and magnitude depended on N availability. Primary roots were longer and root hairs shorter regardless of N, with stronger changes at low N. At higher N, H. seropedicae provided 11% of the total plant N that came from sources other than the seed or the nutrient solution. The time-resolved phenotypic and molecular data point to distinct modes of action: at 5 mM NH₄NO₃ the benefit appears through N fixation, while at 0.5 mM NH₄NO₃ the mechanism appears to be plant physiological, with H. seropedicae promoting uptake of N from the root medium.Future work could fine-tune plant and root-associated microorganisms to growth and nutrient dynamics.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: Forschungszentrum Jülich–University of Melbourne postgraduate academy (JUMPA); German Research Foundation (DFG); Office of the China Postdoctoral Council (OCPC); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1907596

Journal Information:: Journal of Experimental Botany, Journal Name: Journal of Experimental Botany Journal Issue: 15 Vol. 73; ISSN 0022-0957

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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N-dependent dynamics of root growth and nitrate and ammonium uptake are altered by the bacterium Herbaspirillum seropedicae in the cereal model Brachypodium distachyon

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