Screening for Lipids From Marine Microalgae Using Nile Red

Johnson, Zackary I.; Bidigare, Robert R.; Blinebry, Sara K.; Brown, Susan L.; Cullen, John J.; Loftus, Sarah E.; Redalje, Donald G.; Swink, Courtney; Van Mooy, Benjamin A. S.

doi:10.1007/978-3-319-31421-1_382-1

Screening for Lipids From Marine Microalgae Using Nile Red

Other · Sun Feb 05 23:00:00 EST 2017 · Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Production of Fuels and Chemicals

DOI:https://doi.org/10.1007/978-3-319-31421-1_382-1· OSTI ID:2280659

Johnson, Zackary I. ^[1]; Bidigare, Robert R. ^[2]; Blinebry, Sara K. ^[3]; Brown, Susan L. ^[2]; Cullen, John J. ^[4]; Loftus, Sarah E. ^[3]; Redalje, Donald G. ^[5]; Swink, Courtney ^[3]; Van Mooy, Benjamin A. S. ^[6]

Duke University, Durham, NC (United States); Duke University
University of Hawaii, Honolulu, HI (United States)
Duke University, Durham, NC (United States)
Dalhousie University, Halifax, NS (Canada)
University of Southern Mississippi, Hattiesburg, MS (United States); Stennis Space Center, Kiln, MS (United States)
Woods Hole Oceanographic Institution, MA (United States)

The fluorescent stain Nile Red has been used extensively for the quantification of lipids in phytoplankton, including microalgae, because it preferentially stains neutral lipids and it is economical and sensitive to use for screening purposes. Although its basic application has not changed for several decades, recent improvements have been made to improve its utility across applications. Here we describe additional refinements in its application and interpretation as a highthroughput method for the rapid quantification of neutral lipids in liquid cultures of marine phytoplankton. Specifically we address (1) interspecies comparisons, (2) fluorescence excitation and emission wavelengths, and (3) the time course of the Nile Red signal in the context of using bulk or cell-specific fluorescence to quantify neutral lipids oflive or preserved cells. Here, we show that with proper caution in its interpretation across species and physiological states the quantity of lipid in hundreds of small volume samples can be reliably assessed daily using a refined Nile Red protocol.

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Research Organization:: Duke University, Durham, NC (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO)

DOE Contract Number:: EE0007091

OSTI ID:: 2280659

Journal Information:: Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Production of Fuels and Chemicals, Journal Name: Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Production of Fuels and Chemicals

Country of Publication:: United States

Language:: English

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Reused Cultivation Water Accumulates Dissolved Organic Carbon and Uniquely Influences Different Marine Microalgae Loftus, Sarah E.; Johnson, Zackary I. Frontiers in Bioengineering and Biotechnology, Vol. 7 https://doi.org/10.3389/fbioe.2019.00101	journal	May 2019

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Screening for Lipids From Marine Microalgae Using Nile Red

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