Biocompatible Water Soluble Polyacrylic Acid Coated CdSe/Cu Quantum Dot Conjugates for Biomolecule Detection

Journal Article · Mon Jan 15 04:00:00 EST 2018 · Journal of Fluorescence (Online)

DOI:https://doi.org/10.1007/S10895-017-2158-3· OSTI ID:22795690

Okasha, Aly ^[1]; Hosni, Hany M. ^[2]; El-Hag Ali, Amr ^[3]

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1. National Research Center (NRC), Spectroscopy Department (Egypt)
2. National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Solid State Department (Egypt)
3. National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Polymer Chemistry Department (Egypt)

Biocompatible polyacrylic acid functionalized CdSe/Cu quantum dot conjugates were synthesized to be used for biomolecules detection. The study results demonstrate the conjugation of the 2.5-3 nm QD with gram negative bacteria with a low detection limit of 28 cfu/ml. The photoluminescence (PL) intensity was correlated to bacterial count, cellular proteins and exopolysaccharides in the tested samples. Confocal Scanning Laser Microscopy (CSLM) images showed significant QD uptake within the cells, both cytoplasm and DNA were the predominant targeted biomolecules, higher fluorescent uptake was shown in gram negative bacteria than that observed for gram positive bacteria. Moreover, PL showed that there was a distinction between live and dead cells as well as gram negative and gram positive cells. Cell viability was not affected even after 6 days (100% viability) rendering it a non-toxic QD. The method is simple and is performed in a single step within approximately 10 min as compared to multi-step protocols for classical microbial count or fluorescent dye staining. All the above results indicate that the CdSe/Cu-PAA QDs are suitable for biomolecule detection, bio-labeling and bioimaging applications.

• OSTI ID: 22795690
• Journal Information: Journal of Fluorescence (Online), Journal Name: Journal of Fluorescence (Online) Journal Issue: 1 Vol. 28; ISSN 1573-4994
• Country of Publication: United States
• Language: English

Related Subjects

77 NANOSCIENCE AND NANOTECHNOLOGY
BACTERIA
CADMIUM SELENIDES
CYTOPLASM
DNA
FLUORESCENCE
LASERS
MICROSCOPY
PHOTOLUMINESCENCE
PROTEINS
QUANTUM DOTS
SENSITIVITY