Photosensitization of CdSe/ZnS QDs and reliability of assays for reactive oxygen species production.

Journal Article · Fri Jan 01 04:00:00 EST 2010 · Nanoscale

DOI:https://doi.org/10.1039/b9nr00130a· OSTI ID:971488

Cooper, D R; Dimitrijevic, N M; Nadeau, J L

CdSe/ZnS quantum dots (QDs) conjugated to biomolecules that can act as electron donors are said to be 'photosensitized': that is, they are able to oxidize or reduce molecules whose redox potential lies inside their band edges, in particular molecular oxygen and water. This leads to the formation of reactive oxygen species (ROS) and phototoxicity. In this work, we quantify the generation of different forms of ROS from as-synthesized QDs in toluene; water-solubilized, unconjugated QDs; QDs conjugated to the neurotransmitter dopamine; and dopamine alone. Results of indirect fluorescent ROS assays, both in solution and inside cells, are compared with those of spin-trap electron paramagentic resonance spectroscopy (EPR). The effect of these particles on the metabolism of mammalian cells is shown to be dependent upon light exposure and proportional to the amount of ROS generated.

• Research Organization: Argonne National Laboratory (ANL)
• Sponsoring Organization: SC
• DOE Contract Number: AC02-06CH11357
• OSTI ID: 971488
• Report Number(s): ANL/CSE/JA-66024
• Journal Information: Nanoscale, Journal Name: Nanoscale Journal Issue: 2010 Vol. 2
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
77 NANOSCIENCE AND NANOTECHNOLOGY
99 GENERAL AND MISCELLANEOUS
CADMIUM SELENIDES
DOPAMINE
ELECTRON TRANSFER
METABOLISM
PHOTOSENSITIVITY
QUANTUM DOTS
REDOX POTENTIAL
ZINC SULFIDES