Development of a Xanthene-Based Red-Emissive Fluorescent Probe for Visualizing H{sub 2}O{sub 2} in Living Cells, Tissues and Animals
- University of Jinan, Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering (China)
Hydrogen peroxide (H{sub 2}O{sub 2}) plays important roles in the regulation of many biological processes, and the abnormal level of H{sub 2}O{sub 2} has close relation with the initiation and progression of many diseases. Herein, we describe a novel red-emissive fluorescence probe (RhoB) for the visualization of H{sub 2}O{sub 2} in living cells, tissues and animals. RhoB was constructed on the basis of a xanthene-based red-emissive dye, and displayed nearly no fluorescence. After the treatment with H{sub 2}O{sub 2}, RhoB can exhibit red fluorescence with the emission wavelength at 638 nm. RhoB exhibited highly sensitive and selective response to H{sub 2}O{sub 2}. Density functional theory (DFT) calculations were conducted to shed light on the optical properties of RhoB, and natural bond orbital (NBO) calculations demonstrate that the boron atom shows the highest positive electricity and further support the response mechanism. RhoB was successfully applied for imaging of exogenous and endogenous H{sub 2}O{sub 2} in living cells, and also can be utilized for visualizing H{sub 2}O{sub 2} in living tissues and animals.
- OSTI ID:
- 22795641
- Journal Information:
- Journal of Fluorescence (Online), Journal Name: Journal of Fluorescence (Online) Journal Issue: 2 Vol. 28; ISSN 1573-4994
- Country of Publication:
- United States
- Language:
- English
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