Adduct Formation in ESI/MS by Mobile Phase Additives

Journal Article · Mon May 15 04:00:00 EDT 2017 · Journal of the American Society for Mass Spectrometry

DOI:https://doi.org/10.1007/S13361-017-1626-Y· OSTI ID:22776942

Kruve, Anneli; Kaupmees, Karl ^[1]

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1. University of Tartu, Institute of Chemistry (Estonia)

Adduct formation is a common ionization method in electrospray ionization mass spectrometry (ESI/MS). However, this process is poorly understood and complicated to control. We demonstrate possibilities to control adduct formation via mobile phase additives in ESI positive mode for 17 oxygen and nitrogen bases. Mobile phase additives were found to be a very effective measure for manipulating the formation efficiencies of adducts. An appropriate choice of additive may increase sensitivity by up to three orders of magnitude. In general, sodium adduct [M + Na]{sup +} and protonated molecule [M + H]{sup +} formation efficiencies were found to be in good correlation; however, the former were significantly more influenced by mobile phase properties. Although the highest formation efficiencies for both species were observed in water/acetonitrile mixtures not containing additives, the repeatability of the formation efficiencies was found to be improved by additives. It is concluded that mobile phase additives are powerful, yet not limiting factors, for altering adduct formation. .

• OSTI ID: 22776942
• Journal Information: Journal of the American Society for Mass Spectrometry, Journal Name: Journal of the American Society for Mass Spectrometry Journal Issue: 5 Vol. 28; ISSN 1044-0305; ISSN JAMSEF
• Country of Publication: United States
• Language: English

Related Subjects

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
ADDUCTS
CONTROL
CORRELATIONS
EFFICIENCY
IONIZATION
MASS SPECTROSCOPY
MIXTURES
MOLECULES
SENSITIVITY