ESI-MS of protein noncovalent tetrameric associations at high m/z
- Pacific Northwest Lab., Richland, WA (United States); and others
Electrospray ionization mass spectrometry (ESI-MS) has demonstrated broad applicability in the analysis of liquid-phase noncovalent complexes. Successful detection of these complexes by ESI-MS relies on the use of proper solution conditions and careful control of instrumental variables in the atmosphere-vacuum interface region. For large multisubunit proteins, electrospraying from near-neutral pH aqueous solutions, rather than from conventional acidic solutions, is often essential in detecting the biologically active form of the protein. However, the limited gas-phase charging typically observed for these proteins, presumably due to their compact quaternary structures necessitates the use of mass spectrometers capable of extended m/z range detection (>3,000).
- OSTI ID:
- 210738
- Report Number(s):
- CONF-9505261--
- Country of Publication:
- United States
- Language:
- English
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