Collision Cross Sections and Ion Mobility Separation of Fragment Ions from Complex N-Glycans
- University of Southampton, Biological Sciences and the Institute for Life Sciences (United Kingdom)
- The National Institute for Bioprocessing Research and Training, NIBRT GlycoScience Group (Ireland)
- Fritz Haber Institute of the Max Planck Society (Germany)
Ion mobility mass spectrometry (IM-MS) holds great potential for structural glycobiology, in particular in its ability to resolve glycan isomers. Generally, IM-MS has largely been applied to intact glycoconjugate ions with reports focusing on the separation of different adduct types. Here, we explore IM separation and report the collision cross section (CCS) of complex type N-glycans and their fragments in negative ion mode following collision-induced dissociation (CID). CCSs of isomeric fragment ions were found, in some cases, to reveal structural details that were not present in CID spectra themselves. Many fragment ions were confirmed as possessing multiple structure, details of which could be obtained by comparing their drift time profiles to different glycans. By using fragmentation both before and after mobility separation, information was gathered on the fragmentation pathways producing some of the ions. These results help demonstrate the utility of IM and will contribute to the growing use of IM-MS for glycomics. .
- OSTI ID:
- 22776900
- Journal Information:
- Journal of the American Society for Mass Spectrometry, Vol. 29, Issue 6; Other Information: Copyright (c) 2018 American Society for Mass Spectrometry; http://www.springer-ny.com; Country of input: International Atomic Energy Agency (IAEA); ISSN 1044-0305
- Country of Publication:
- United States
- Language:
- English
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