Structural Studies of Fucosylated N-Glycans by Ion Mobility Mass Spectrometry and Collision-Induced Fragmentation of Negative Ions

Journal Article · Fri Jun 15 04:00:00 EDT 2018 · Journal of the American Society for Mass Spectrometry

DOI:https://doi.org/10.1007/S13361-018-1950-X· OSTI ID:22776898

There is considerable potential for the use of ion mobility mass spectrometry in structural glycobiology due in large part to the gas-phase separation attributes not typically observed by orthogonal methods. Here, we evaluate the capability of traveling wave ion mobility combined with negative ion collision-induced dissociation to provide structural information on N-linked glycans containing multiple fucose residues forming the Lewis{sup x} and Lewis{sup y} epitopes. These epitopes are involved in processes such as cell-cell recognition and are important as cancer biomarkers. Specific information that could be obtained from the intact N-glycans by negative ion CID included the general topology of the glycan such as the presence or absence of a bisecting GlcNAc residue and the branching pattern of the triantennary glycans. Information on the location of the fucose residues was also readily obtainable from ions specific to each antenna. Some isobaric fragment ions produced prior to ion mobility could subsequently be separated and, in some cases, provided additional valuable structural information that was missing from the CID spectra alone. .

• OSTI ID: 22776898
• Journal Information: Journal of the American Society for Mass Spectrometry, Journal Name: Journal of the American Society for Mass Spectrometry Journal Issue: 6 Vol. 29; ISSN 1044-0305; ISSN JAMSEF
• Country of Publication: United States
• Language: English

Related Subjects

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
74 ATOMIC AND MOLECULAR PHYSICS
BIOLOGICAL MARKERS
BRANCHING RATIO
DISSOCIATION
FRAGMENTATION
ION COLLISIONS
ION MOBILITY
MASS SPECTROSCOPY
NEOPLASMS
SPECTRA