Deregulation of miR-126 expression in colorectal cancer pathogenesis and its clinical significance

Journal Article · Thu Dec 10 04:00:00 EST 2015 · Experimental Cell Research

DOI:https://doi.org/10.1016/J.YEXCR.2015.10.004· OSTI ID:22746387

Ebrahimi, Faeza ^[1]; Gopalan, Vinod ^[1]; Wahab, Riajul ^[1]; Lu, Cu-Tai ^[2]; Anthony Smith, Robert ^[1]

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1. Cancer Molecular Pathology, School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast, Queensland (Australia)
2. Department of Surgery, Gold Coast Hospital, Gold Coast, Queensland (Australia)

In this study, we investigated the expression profiles and clinicopathological significance of miR-126 in large cohort of patients with colorectal cancers as well the cellular repercussions of miR-126 in colon cancer cells along with its targets in-vitro. Down regulation of miR-126 expression was associated with histological subtypes, peri-neural tumour infiltration, microsatellite instability and pathological staging of colorectal cancers (p<0.05). Low miR-126 expression was also associated with poorer survival in patients with colorectal cancer. Analysis of matched tissues from the same patient revealed that approximately 70% of the tested patients had similar levels of expression of miR-126 in primary cancer and cancer metastases in both lymph node and distant metastases. In addition, induced overexpression of miR-126 showed reduced cell proliferation, increased apoptosis and decreased accumulation of cells in the G{sub 0}–G{sub 1} phase of the colon cancer cells. Furthermore, SW480{sup +miR-126} cells showed reduced BCL-2 and increased P53 protein expression. To conclude, deregulation of miR-126 in colorectal cancer at the tissue and cellular levels as well as its correlation with various clinicopathological parameters confirm the cancer suppressive role of miR-126 in colorectal cancer. - Highlights: • Low miR-126 expression was associated with pathological features, MSI and staging. • Low miR-126 expression was associated with poorer patients’ survival in CRC. • Many patients had similar levels of miR-126 expression in primary cancer and cancer metastases. • Induced miR-126 expression reduces cancer proliferation & increases apoptosis. • SW480{sup +miR-126} cells showed reduced BCL-2 and increased P53 protein expression.

• OSTI ID: 22746387
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 2 Vol. 339; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANIMAL TISSUES
APOPTOSIS
APPROXIMATIONS
BORON CHLORIDES
CELL PROLIFERATION
IN VITRO
LARGE INTESTINE
LYMPH
LYMPH NODES
METASTASES
NEOPLASMS
PATHOGENESIS
PATIENTS
PROTEINS