skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cytoprotection, proliferation and epidermal differentiation of adipose tissue-derived stem cells on emu oil based electrospun nanofibrous mat

Abstract

Electrospun nanofibrous scaffolds containing natural substances with wound healing properties such as Emu oil (EO) may have a great potential for increasing the efficiency of stem cell-based skin bioengineering. For this purpose, EO blended PCL/PEG electrospun nanofibrous mats were successfully fabricated and characterized using FE-SEM, FTIR and Universal Testing Machine. The efficiency of the scaffolds in supporting the adherence, cytoprotection, proliferation and differentiation of adipose tissue-derived stem cells (ADSCs) to keratinocyte was evaluated. GC/MS and HPLC were used to determine the composition of pure EO, which revealed to be mainly fatty acids and carotenoids. FE-SEM and cell proliferation assays showed that adhesion and proliferation of ADSCs on EO-PCL/PEG nanofibers was significantly higher than on PCL/PEG nanofibers. Additionally, EO-PCL/PEG nanofibers with free radical scavenging properties conferred a cytoprotective effect against cell-damaging free radicals, while the ability to support cell adhesion and growth was maintained or even improved. Immunostaining of ADSCs on EO-PCL/PEG nanofibers confirmed the change in morphology of ADSCs from spindle to polygonal shape suggesting their differentiation toward an epidermal linage. Moreover, the expression levels of the keratin 10, filaggrin, and involucrin that are involved in epidermal differentiation were upregulated in a stage-specific manner. This preliminary study shows that EO-PCL/PEGmore » nanofibers could be a good candidate for the fabrication of wound dressings and skin bioengineered substitutes with ADSCs. - Highlights: • EO-PCL/PEG nanofibers showed excellent biocompatibility. • EO-PCL/PEG nanofibers conferred a cytoprotective effect against free radicals. • EO with nanofibrous scaffolds promoted epidermal differentiation of ADSCs. • EO-PCL/PEG with ADSCs provide an efficient cell–scaffold construct.« less

Authors:
 [1];  [2];  [3];  [1];  [2];  [4];  [5];  [1]; ;  [5];  [1];  [2]
  1. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz (Iran, Islamic Republic of)
  2. (Iran, Islamic Republic of)
  3. (Australia)
  4. Australian Regenerative Medicine Institute, Monash University, Clayton 3800 (Australia)
  5. Department of Clinical Biochemistry and Laboratory Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz (Iran, Islamic Republic of)
Publication Date:
OSTI Identifier:
22738144
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 357; Journal Issue: 2; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADIPOSE TISSUE; CARBOXYLIC ACIDS; CELL PROLIFERATION; EFFICIENCY; FOURIER TRANSFORMATION; GAS CHROMATOGRAPHY; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; INFRARED SPECTRA; MASS SPECTROSCOPY; NANOFIBERS; OILS; RADICALS; STEM CELLS

Citation Formats

Pilehvar-Soltanahmadi, Younes, Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Australian Regenerative Medicine Institute, Monash University, Clayton 3800, Nouri, Mohammad, Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Martino, Mikaël M., Fattahi, Amir, Alizadeh, Effat, Darabi, Masoud, Rahmati-Yamchi, Mohammad, Zarghami, Nosratollah, and Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz. Cytoprotection, proliferation and epidermal differentiation of adipose tissue-derived stem cells on emu oil based electrospun nanofibrous mat. United States: N. p., 2017. Web. doi:10.1016/J.YEXCR.2017.05.015.
Pilehvar-Soltanahmadi, Younes, Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Australian Regenerative Medicine Institute, Monash University, Clayton 3800, Nouri, Mohammad, Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Martino, Mikaël M., Fattahi, Amir, Alizadeh, Effat, Darabi, Masoud, Rahmati-Yamchi, Mohammad, Zarghami, Nosratollah, & Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz. Cytoprotection, proliferation and epidermal differentiation of adipose tissue-derived stem cells on emu oil based electrospun nanofibrous mat. United States. doi:10.1016/J.YEXCR.2017.05.015.
Pilehvar-Soltanahmadi, Younes, Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Australian Regenerative Medicine Institute, Monash University, Clayton 3800, Nouri, Mohammad, Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Martino, Mikaël M., Fattahi, Amir, Alizadeh, Effat, Darabi, Masoud, Rahmati-Yamchi, Mohammad, Zarghami, Nosratollah, and Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz. Tue . "Cytoprotection, proliferation and epidermal differentiation of adipose tissue-derived stem cells on emu oil based electrospun nanofibrous mat". United States. doi:10.1016/J.YEXCR.2017.05.015.
@article{osti_22738144,
title = {Cytoprotection, proliferation and epidermal differentiation of adipose tissue-derived stem cells on emu oil based electrospun nanofibrous mat},
author = {Pilehvar-Soltanahmadi, Younes and Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz and Australian Regenerative Medicine Institute, Monash University, Clayton 3800 and Nouri, Mohammad and Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz and Martino, Mikaël M. and Fattahi, Amir and Alizadeh, Effat and Darabi, Masoud and Rahmati-Yamchi, Mohammad and Zarghami, Nosratollah and Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz},
abstractNote = {Electrospun nanofibrous scaffolds containing natural substances with wound healing properties such as Emu oil (EO) may have a great potential for increasing the efficiency of stem cell-based skin bioengineering. For this purpose, EO blended PCL/PEG electrospun nanofibrous mats were successfully fabricated and characterized using FE-SEM, FTIR and Universal Testing Machine. The efficiency of the scaffolds in supporting the adherence, cytoprotection, proliferation and differentiation of adipose tissue-derived stem cells (ADSCs) to keratinocyte was evaluated. GC/MS and HPLC were used to determine the composition of pure EO, which revealed to be mainly fatty acids and carotenoids. FE-SEM and cell proliferation assays showed that adhesion and proliferation of ADSCs on EO-PCL/PEG nanofibers was significantly higher than on PCL/PEG nanofibers. Additionally, EO-PCL/PEG nanofibers with free radical scavenging properties conferred a cytoprotective effect against cell-damaging free radicals, while the ability to support cell adhesion and growth was maintained or even improved. Immunostaining of ADSCs on EO-PCL/PEG nanofibers confirmed the change in morphology of ADSCs from spindle to polygonal shape suggesting their differentiation toward an epidermal linage. Moreover, the expression levels of the keratin 10, filaggrin, and involucrin that are involved in epidermal differentiation were upregulated in a stage-specific manner. This preliminary study shows that EO-PCL/PEG nanofibers could be a good candidate for the fabrication of wound dressings and skin bioengineered substitutes with ADSCs. - Highlights: • EO-PCL/PEG nanofibers showed excellent biocompatibility. • EO-PCL/PEG nanofibers conferred a cytoprotective effect against free radicals. • EO with nanofibrous scaffolds promoted epidermal differentiation of ADSCs. • EO-PCL/PEG with ADSCs provide an efficient cell–scaffold construct.},
doi = {10.1016/J.YEXCR.2017.05.015},
journal = {Experimental Cell Research},
issn = {0014-4827},
number = 2,
volume = 357,
place = {United States},
year = {2017},
month = {8}
}