Intracellular chloride ion concentration in differentiating neuronal cell and its role in growing neurite

Journal Article · Fri Oct 14 04:00:00 EDT 2016 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2016.09.075· OSTI ID:22696648

Nakajima, Ken-ichi ^[1]; Marunaka, Yoshinori ^[1]

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1. Department of Molecular Cell Physiology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto 602-8566 (Japan)

Chloride ion (Cl{sup −}) is one of the most abundant anions in our body. Increasing evidence suggests that Cl{sup −} plays fundamental roles in various cellular functions. We have previously reported that electroneutral cation-chloride cotransporters, such as Na{sup +}-K{sup +}-2Cl{sup −} cotransporter 1 (NKCC1) and K{sup +}-Cl{sup −} cotransporter 1 (KCC1), are involved in neurite outgrowth during neuronal differentiation. In the present study, we studied if there is correlation between intracellular Cl{sup −} concentrations ([Cl{sup −}]{sub i}) and the length of growing neurites. We measured [Cl{sup −}]{sub i} in the cell body and growing neurite tips using halide-sensitive fluorescent dye N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE), revealing that [Cl{sup −}]{sub i} in the tip of growing neurite was higher than that in cell body in a single cell. Importantly, there was a significant positive correlation between the length of growing neurite and [Cl{sup −}]{sub i} in neurite tip. Bumtanide (BMT), an inhibitor of NKCC1, significantly inhibited neurite outgrowth and decreased [Cl{sup −}]{sub i} in neurite tip. The results obtained in the present study and our previous studies together strongly suggest that high [Cl{sup −}]{sub i} in neurite tip region is crucial for efficient neurite outgrowth. - Highlights: • Intracellular Cl{sup −} concentrations ([Cl{sup −}]{sub i}) in the tip of growing neurite is higher than that in cell body in a single cell. • There is a significant positive correlation between the length of growing neurite and [Cl{sup −}]{sub i} in neurite tip. • Bumetanide significantly inhibits neurite outgrowth and decreased [Cl{sup −}]{sub i} in neurite tip. • High [Cl{sup −}]{sub i} in neurite tip region is crucial for efficient neurite outgrowth.

• OSTI ID: 22696648
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 479; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CHLORIDES
CHLORINE IONS
CONCENTRATION RATIO
CORRELATIONS
ECOLOGICAL CONCENTRATION
INHIBITION
LENGTH