Essential role of NKCC1 in NGF-induced neurite outgrowth

Journal Article · Fri Aug 03 04:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.05.133· OSTI ID:20991470

Nakajima, Ken-ichi ^[1]; Miyazaki, Hiroaki ^[1]; Niisato, Naomi ^[1]; Marunaka, Yoshinori ^[2]

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1. Department of Molecular Cell Physiology, Graduate School of Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566 (Japan)
2. Department of Molecular Cell Physiology, Graduate School of Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566 (Japan) and Department of Respiratory Medicine, Graduate School of Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566 (Japan)

The Na{sup +}/K{sup +}/2Cl{sup -} cotransporter (NKCC) mediates electroneutral transport of 2Cl{sup -} coupled with Na{sup +} and K{sup +} across the plasma membrane, and plays crucial roles in Cl{sup -} uptake into the cells, homeostasis of cellular Cl{sup -}, and cell volume regulation. However, we have very limited information on the roles of ion transporters in neurite outgrowth in neuronal cells. In the present study, we report the role of NKCC1 (an isoform of NKCC) in NGF-induced neurite outgrowth of rat pheochromocytoma PC12D cells. The expression level of NKCC1 protein was increased by NGF treatment. Knock-down of NKCC1 by RNA interference (RNAi) drastically diminished the NGF-induced neurite outgrowth. Transfection of enhanced green fluorescent protein (EGFP)-tagged rat NKCC1 into cells for clarification of intracellular localization of NKCC1 revealed that the EGFP-rNKCC1 was mainly localized in the plasma membrane at growth cone during neurite outgrowth. These observations suggest that NKCC1 plays a fundamental role in NGF-induced neurite outgrowth of PC12D cells.

• OSTI ID: 20991470
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 359; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL MEMBRANES
CHLORINE IONS
GENE REGULATION
HOMEOSTASIS
INFORMATION
POTASSIUM IONS
PROTEINS
RATS
RNA
SODIUM IONS
UPTAKE