In-situ data collection at the photon factory macromolecular crystallography beamlines
- Structural Biology Research Center, Institute of Materials Structure Science, KEK (High Energy Accelerator Research Organization), 1-1 Oho, Tsukuba, Ibaraki (Japan)
- Mechanical Engineering Center, Applied Research Laboratory, KEK, 1-1 Oho, Tsukuba, Ibaraki (Japan)
Crystallization trial is one of the most important but time-consuming steps in macromolecular crystallography, and in-situ diffraction experiment has a capability to make researchers to proceed this step more efficiently. At the Photon Factory, a new tabletop diffractometer for in-situ diffraction experiments has been developed. It consists of XYZ translation stages with a plate handler, an on-axis viewing system and a plate rack with a capacity for ten crystallization plates. These components sit on a common plate and are able to be placed on the existing diffractometer table. The CCD detector with a large active area and a pixel array detector with a small active area are used for acquiring diffraction images from crystals. Dedicated control software and a user interface have also been developed. The new diffractometer has been operational for users and used for evaluation of crystallization screening since 2014.
- OSTI ID:
- 22608447
- Journal Information:
- AIP Conference Proceedings, Journal Name: AIP Conference Proceedings Journal Issue: 1 Vol. 1741; ISSN 0094-243X; ISSN APCPCS
- Country of Publication:
- United States
- Language:
- English
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