The voltage-gated proton channel Hv1 is expressed in pancreatic islet β-cells and regulates insulin secretion

Journal Article · Fri Dec 25 04:00:00 EST 2015 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2015.11.027· OSTI ID:22594148

Zhao, Qing ^[1]; Che, Yongzhe ^[2]; Li, Qiang; Zhang, Shangrong ^[1]; Gao, Ying-Tang ^[3]; Wang, Yifan; Wang, Xudong; Xi, Wang; Zuo, Weiyan ^[1]

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1. Department of Biophysics, School of Physics Science, The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071 (China)
2. School of Medicine, Nankai University, Tianjin 300071 (China)
3. Key Laboratory of Artificial Cell, Third Central Clinical College of Tianjin Medical University, Tianjin 300170 (China)

The voltage-gated proton channel Hv1 is a potent acid extruder that participates in the extrusion of the intracellular acid. Here, we showed for the first time, Hv1 is highly expressed in mouse and human pancreatic islet β-cells, as well as β-cell lines. Imaging studies demonstrated that Hv1 resides in insulin-containing granules in β-cells. Knockdown of Hv1 with RNA interference significantly reduces glucose- and K{sup +}-induced insulin secretion in isolated islets and INS-1 (832/13) β-cells and has an impairment on glucose- and K{sup +}-induced intracellular Ca{sup 2+} homeostasis. Our data demonstrated that the expression of Hv1 in pancreatic islet β-cells regulates insulin secretion through regulating Ca{sup 2+} homeostasis.

• OSTI ID: 22594148
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 468; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BIOMEDICAL RADIOGRAPHY
CALCIUM IONS
GLUCOSE
HOMEOSTASIS
INSULIN
MICE
PANCREAS
POTASSIUM IONS
RNA