Gastrin releasing peptide augments glucose mediated 45Ca2+ uptake, electrical activity, and insulin secretion of mouse pancreatic islets

Journal Article · Sat Jun 01 04:00:00 EDT 1991 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-128-6-3247· OSTI ID:5596098

Wahl, M A; Plehn, R J; Landsbeck, E A; Verspohl, E J; Ammon, H P ^[1]

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1. Department of Pharmacology, Eberhard-Karls Universitaet Tuebingen (West Germany)

Gastrin releasing peptide (GRP) has recently been shown to increase glucose-induced insulin secretion in vivo. Being present in pancreatic tissue, the 27-amino acid peptide could play a role in the control of the glucose-induced insulin secretion of islets of Langerhans. In the presence of a stimulatory glucose concentration, GRP augmented insulin secretion of isolated islets in batch incubations. The peptide did not affect 86Rb+ efflux in the presence of 3 or 5.6 mM glucose but reduced the increase of 86Rb+ efflux evoked by the calcium ionophore A23187. 45Ca2+ uptake and intracellular recorded electrical activity induced by glucose were amplified by GRP. It is suggested that GRP plays a role in the regulation of glucose-induced insulin secretion by increasing the uptake of Ca2+ directly or by inhibition of the Ca(2+)-dependent K+ channel activity and reduced repolarization of the cell.

• OSTI ID: 5596098
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 128:6; ISSN ENDOA; ISSN 0013-7227
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHYSIOLOGY
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
GLUCOSE
HEXOSES
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE TRANSPORT
MICE
MINUTES LIVING RADIOISOTOPES
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDE HORMONES
PEPTIDES
PHYSIOLOGY
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOP
SACCHARIDES
SECRETION
TRACER TECHNIQUES
VERTEBRATES