Maintenance of sweat glands by stem cells located in the acral epithelium

Journal Article · Fri Oct 23 04:00:00 EDT 2015 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2015.09.022· OSTI ID:22592762

Ohe, Shuichi ^[1]; Tanaka, Toshihiro ^[1]; Yanai, Hirotsugu ^[1]; Komai, Yoshihiro ^[1]; Omachi, Taichi ^[1]; Kanno, Shohei; Tanaka, Kiyomichi; Ishigaki, Kazuhiko; Saiga, Kazuho ^[1]; Nakamura, Naohiro ^[1]; Ohsugi, Haruyuki ^[1]; Tokuyama, Yoko; Atsumi, Naho; Hisha, Hiroko; Yoshida, Naoko; Kumano, Keiki ^[1]; Yamazaki, Fumikazu; Okamoto, Hiroyuki ^[2]; Ueno, Hiroo ^[1]

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1. Department of Stem Cell Pathology, Kansai Medical University, Osaka 573-1010 (Japan)
2. Department of Dermatology, Kansai Medical University, Osaka 573-1010 (Japan)

The skin is responsible for a variety of physiological functions and is critical for wound healing and repair. Therefore, the regenerative capacity of the skin is important. However, stem cells responsible for maintaining the acral epithelium had not previously been identified. In this study, we identified the specific stem cells in the acral epithelium that participate in the long-term maintenance of sweat glands, ducts, and interadnexal epidermis and that facilitate the regeneration of these structures following injury. Lgr6-positive cells and Bmi1-positive cells were found to function as long-term multipotent stem cells that maintained the entire eccrine unit and the interadnexal epidermis. However, while Lgr6-positive cells were rapidly cycled and constantly supplied differentiated cells, Bmi1-positive cells were slow to cycle and occasionally entered the cell cycle under physiological conditions. Upon irradiation-induced injury, Bmi1-positive cells rapidly proliferated and regenerated injured epithelial tissue. Therefore, Bmi1-positive stem cells served as reservoir stem cells. Lgr5-positive cells were rapidly cycled and maintained only sweat glands; therefore, we concluded that these cells functioned as lineage-restricted progenitors. Taken together, our data demonstrated the identification of stem cells that maintained the entire acral epithelium and supported the different roles of three cellular classes. - Highlights: • The acral epithelium have two types of stem cells. • Lgr6-positive cells are rapid-cycling, short-term stem cells. • Bmi1-positive cells are slow-cycling stem cells that act as reserver stem cells. • Lgr5 may be a useful sweat gland marker in mice.

• OSTI ID: 22592762
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 466; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL CYCLE
EPIDERMIS
GLANDS
GTP-ASES
HEALING
IRRADIATION
LEUCINE
LYMPHOMAS
MICE
RECEPTORS
STEM CELLS
SWEAT
WOUNDS