Diffusion studies on permeable nitroxyl spin probes through bilayer lipid membranes: A low frequency ESR study
- Department of Physics, NMSSVN College, Nagamalai, Madurai-625019, Tamilnadu (India)
- Department of Bio-functional Science, Kyushu University, Fukuoka (Japan)
- Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka (Japan)
- Department of Chemistry, NMSSVN College, Nagamalai, Madurai-625019, Tamilnadu (India)
Electron spin resonance (ESR) studies were carried out for permeable 2mM {sup 14}N-labeled deutrated 3 Methoxy carbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 1mM, 2mM, 3mM, 4mM concentration of 14N-labeled deutrated MC-PROXYL in 400mM concentration of liposomal solution by using a 300 MHz ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported for these samples. The line broadening was observed for the nitroxyl spin probe in the liposomal solution. The line broadening indicates that the high viscous nature of the liposomal solution. The partition parameter and permeability values indicate the maximum diffusion of nitroxyl spin probes in the bilayer lipid membranes at 2 mM concentration of nitroxyl radical. This study illustrates that ESR can be used to differentiate between the intra and extra- membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the spin probe concentration was optimized as 2mM in liposomal solution for ESR phantom studies/imaging, invivo and invitro experiments.
- OSTI ID:
- 22490217
- Journal Information:
- AIP Conference Proceedings, Journal Name: AIP Conference Proceedings Journal Issue: 1 Vol. 1665; ISSN APCPCS; ISSN 0094-243X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Permeability studies of redox-sensitive nitroxyl spin probes in corn oil using an L-band ESR spectrometer
Reduction process of nitroxyl spin probes used in Overhauser-enhanced magnetic resonance imaging: An ESR study