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Title: Isolation and characterization of a novel arenavirus harbored by Rodents and Shrews in Zhejiang province, China

Abstract

To determine the biodiversity of arenaviruses in China, we captured and screened rodents and shrews in Wenzhou city, Zhejiang province, a locality where hemorrhagic fever diseases are endemic in humans. Accordingly, arenaviruses were detected in 42 of 351 rodents from eight species, and in 12 of 272 Asian house shrews (Suncus murinus), by RT-PCR targeting the L segment. From these, a single arenavirus was successfully isolated in cell culture. The virion particles exhibited a typical arenavirus morphology under transmission electron microscopy. Comparison of the S and L segment sequences revealed high levels of nucleotide (>32.2% and >39.6%) and amino acid (>28.8% and >43.8%) sequence differences from known arenaviruses, suggesting that it represents a novel arenavirus, which we designated Wenzhou virus (WENV). Phylogenetic analysis revealed that all WENV strains harbored by both rodents and Asian house shrews formed a distinct lineage most closely related to Old World arenaviruses. - Highlights: • A novel arenavirus (Wenzhou virus) was identified in Zhejiang province, China. • The virus is highly circulating in five species of rats and one species of shrews • More efforts are needed to infer whether it is pathogenic to humans or not.

Authors:
 [1];  [2];  [3];  [1];  [2];  [1];  [2];  [1];  [2];  [3];  [4];
  1. State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing (China)
  2. (China)
  3. Wenzhou Center for Disease Control and Prevention, Wenzhou, Zhejiang Province (China)
  4. Wencheng Center for Disease Control and Prevention, Wenzhou, Zhejiang Province (China)
Publication Date:
OSTI Identifier:
22470148
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 476; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACIDS; CAPTURE; CELL CULTURES; CHINA; COMPARATIVE EVALUATIONS; FEVER; HUMAN POPULATIONS; MORPHOLOGY; NUCLEOTIDES; RATS; SHREWS; SPECIES DIVERSITY; TRANSMISSION; TRANSMISSION ELECTRON MICROSCOPY; VIRUSES

Citation Formats

Li, Kun, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Lin, Xian-Dan, Wang, Wen, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Shi, Mang, Wencheng Center for Disease Control and Prevention, Wenzhou, Zhejiang Province, Guo, Wen-Ping, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhang, Xiao-He, Xing, Jian-Guang, and and others. Isolation and characterization of a novel arenavirus harbored by Rodents and Shrews in Zhejiang province, China. United States: N. p., 2015. Web. doi:10.1016/J.VIROL.2014.11.026.
Li, Kun, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Lin, Xian-Dan, Wang, Wen, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Shi, Mang, Wencheng Center for Disease Control and Prevention, Wenzhou, Zhejiang Province, Guo, Wen-Ping, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhang, Xiao-He, Xing, Jian-Guang, & and others. Isolation and characterization of a novel arenavirus harbored by Rodents and Shrews in Zhejiang province, China. United States. doi:10.1016/J.VIROL.2014.11.026.
Li, Kun, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Lin, Xian-Dan, Wang, Wen, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Shi, Mang, Wencheng Center for Disease Control and Prevention, Wenzhou, Zhejiang Province, Guo, Wen-Ping, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhang, Xiao-He, Xing, Jian-Guang, and and others. Sun . "Isolation and characterization of a novel arenavirus harbored by Rodents and Shrews in Zhejiang province, China". United States. doi:10.1016/J.VIROL.2014.11.026.
@article{osti_22470148,
title = {Isolation and characterization of a novel arenavirus harbored by Rodents and Shrews in Zhejiang province, China},
author = {Li, Kun and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou and Lin, Xian-Dan and Wang, Wen and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou and Shi, Mang and Wencheng Center for Disease Control and Prevention, Wenzhou, Zhejiang Province and Guo, Wen-Ping and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou and Zhang, Xiao-He and Xing, Jian-Guang and and others},
abstractNote = {To determine the biodiversity of arenaviruses in China, we captured and screened rodents and shrews in Wenzhou city, Zhejiang province, a locality where hemorrhagic fever diseases are endemic in humans. Accordingly, arenaviruses were detected in 42 of 351 rodents from eight species, and in 12 of 272 Asian house shrews (Suncus murinus), by RT-PCR targeting the L segment. From these, a single arenavirus was successfully isolated in cell culture. The virion particles exhibited a typical arenavirus morphology under transmission electron microscopy. Comparison of the S and L segment sequences revealed high levels of nucleotide (>32.2% and >39.6%) and amino acid (>28.8% and >43.8%) sequence differences from known arenaviruses, suggesting that it represents a novel arenavirus, which we designated Wenzhou virus (WENV). Phylogenetic analysis revealed that all WENV strains harbored by both rodents and Asian house shrews formed a distinct lineage most closely related to Old World arenaviruses. - Highlights: • A novel arenavirus (Wenzhou virus) was identified in Zhejiang province, China. • The virus is highly circulating in five species of rats and one species of shrews • More efforts are needed to infer whether it is pathogenic to humans or not.},
doi = {10.1016/J.VIROL.2014.11.026},
journal = {Virology},
number = ,
volume = 476,
place = {United States},
year = {Sun Feb 15 00:00:00 EST 2015},
month = {Sun Feb 15 00:00:00 EST 2015}
}