Effective delivery of recombinant proteins to rod photoreceptors via lipid nanovesicles

Journal Article · Fri Jun 12 04:00:00 EDT 2015 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2015.04.088· OSTI ID:22462081

Asteriti, Sabrina ^[1]; Dal Cortivo, Giuditta ^[2]; Pontelli, Valeria ^[3]; Cangiano, Lorenzo ^[1]; Buffelli, Mario ^[3]; Dell’Orco, Daniele ^[2]

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1. Dept. of Translational Research, University of Pisa, Pisa (Italy)
2. Dept. of Life Sciences and Reproduction, University of Verona, Strada Le Grazie 8, Verona (Italy)
3. Dept. of Neurological and Movement Sciences, University of Verona, Strada Le Grazie 8, Verona (Italy)

The potential of liposomes to deliver functional proteins in retinal photoreceptors and modulate their physiological response was investigated by two experimental approaches. First, we treated isolated mouse retinas with liposomes encapsulating either recoverin, an important endogenous protein operating in visual phototransduction, or antibodies against recoverin. We then intravitrally injected in vivo liposomes encapsulating either rhodamin B or recoverin and we investigated the distribution in retina sections by confocal microscopy. The content of liposomes was found to be released in higher amount in the photoreceptor layer than in the other regions of the retina and the functional effects of the release were in line with the current model of phototransduction. Our study sets the basis for quantitative investigations aimed at assessing the potential of intraocular protein delivery via biocompatible nanovesicles, with promising implications for the treatment of retinal diseases affecting the photoreceptor layer. - Highlights: • Recombinant proteins encapsulated in nano-sized liposomes injected intravitreally reach retinal photoreceptors. • The phototransduction cascade in rods is modulated by the liposome content. • Mathematical modeling predicts the alteration of the photoresponses following liposome fusion.

• OSTI ID: 22462081
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 461; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIBODIES
DELIVERY
DISEASES
DISTRIBUTION
IN VIVO
LAYERS
LIPIDS
LIPOSOMES
MATHEMATICAL MODELS
MICE
MICROSCOPY
NANOSTRUCTURES
PROTEINS
RETINA
RODS
THERAPY