Characterization of actions of octanoate on porcine preadipocytes and adipocytes differentiated in vitro

Journal Article · Fri Mar 01 04:00:00 EST 2013 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2013.01.080· OSTI ID:22416597

Suzuki, Shunichi ^[1]; Suzuki, Misae; Sembon, Shoichiro; Fuchimoto, Daiichiro; Onishi, Akira ^[1]

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1. Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba, Ibaraki 305-0901 (Japan)

Highlights: ► Octanoate regulated gene expressions in a way distinct from rosiglitasone. ► Octanoate upregulatedPPRE and LXRE reporter activities. ► Octanoate may act on some PPARγ-target genes competitively with other ligands. - Abstract: Octanoate is used to induce adipogenic differentiation and/or lipid accumulation in preadipocytes of domestic animals. However, information on detailed actions of octanoate and the characteristics of octanoate-induced adipocytes is limited. The aim of this study was to examine these issues by comparing the outcomes of the effects of octanoate with those of rosiglitazone, which is a well-defined activator of peroxisome proliferator-activated receptor (PPAR)-γ. The adipocytes that were differentiated with 5 mM of octanoate had dispersed and diversely sized lipid droplets compared to those that were differentiated with 1 μM of rosiglitazone. The gene expression levels of adiponectin, glycerol-3-phosphate dehydrogenase, perilipin 1, and perilipin 4 were much higher in the adipocytes that were differentiated with rosiglitazone than in those differentiated with octanoate, while the gene expression levels of lipoprotein lipase and perilipin 2 were decreased in rosiglitazone-differentiated adipocytes compared to octanoate-differentiated adipocytes. However, the expressions of aP2 and CD36 genes were comparably induced. Luciferase reporter assays revealed that PPAR and liver-X-receptor activities were upregulated by octanoate more effectively than by rosiglitazone. Overall, these results suggested that the action of octanoate was complicated and may be dependent on the targeted genes and cellular status.

• OSTI ID: 22416597
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 432; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BUILDUP
COMPARATIVE EVALUATIONS
DROPLETS
GENES
GLYCEROL
IN VITRO
LIGANDS
LIPASES
LIPOPROTEINS
LIVER
LUCIFERASE
PHOSPHATES
RECEPTORS
SWINE